Affinity chromatography using agarose-bound lectins was used to isolate erythropoietin from crude preparations of sheep plasma and human urinary erythropoietin. On the basis of previous estimates of the sugar content of the hormone, six lectins (wheat germ agglutinin, phytohemagglutinin, Ricinus communis 120, soybean agglutinin, concanavalin A, and limulin) were chosen for study. Only wheat ger...

Lymphoproliferative responses of tonsillar tissue lymphocytes and peripheral blood lymphocytes to phytohemagglutinin and specific bacterial product antigens were studied in children undergoing tonsillectomy and adenoidectomy. Tonsillar tissue lymphocytes responded to optimal concentrations of phytohemagglutinin. Varidase, and streptolysin-O in a manner similar to peripheral blood lymphocytes. H...

By Douci &ss C. TORMEY AND GERALD C. MUELLER T HE ADDITION of phytohemagglutinin, an extract of the kidney bean Phaseolus vulgaris, to cultures of human peripheral lymphocytes promotes the synthesis of v-globulin, ribonucleic acid (RNA), and desoxyribonucleic acid (DNA), and induces mitosis in a large fraction of lymphocytes.1’7 The present communication describes an assay in which the graded i...

Cytogenetic studies of 222 metaphase lymphocytes stimu lated by phytohemagglutinin were carried out on a patient diagnosed clinically as having Sézarysyndrome. Twenty-two cells (10%) contained 42 to 100 chromosomes. The remaining 200 cells contained 46 chromosomes and revealed evidence of clone formation; 45 were apparently normal diploid cells, but 155 were pseudodiploid with at least one lon...

Cytogenetic studies of 222 metaphase lymphocytes stimulated by phytohemagglutinin were carried out on a patient diagnosed clinically as having Sézary syndrome. Twenty-two cells (10%) contained 42 to 100 chromosomes. The remaining 200 cells contained 46 chromosomes and revealed evidence of clone formation; 45 were apparently normal diploid cells, but 155 were pseudodiploid with at least one long...

Lymphocytes from 14 BCG-vaccinated donors, seven tuberculin positive and seven tuberculin negative by skin testing, were stimulated in vitro with four mycobacterial antigens, purified protein derivative (PPD), PPD/BCG, whole BCG bacilli, and whole Mycobacterium leprae and also with Candida antigen and phytohemagglutinin. The response was measured by incorporation of (3)H-labeled thymidine. The ...