background diabetes mellitus as a chronic metabolic disease occurs in patients with partial or complete deficiency of insulin secretion or disorder in action of insulin on tissue. the disease is known to provide conditions for overgrowth of candida species. candida spp. cause candidiasis by many virulence factors such as esterase, hemolysin and phospholipase. objectives this study aimed to comp...

Chemical modification and site-specific mutagenesis approaches were used in this study to identify the active site serine residue of pancreatic cholesterol esterase. In the first approach, purified porcine pancreatic cholesterol esterase was covalently modified by incubation with [3H]diisopropylfluorophosphate (DFP). The radiolabeled cholesterol esterase was digested with CNBr, and the peptides...

background: gp41 of hiv (human immunodeficiency virus) is a protein that mediates fusion between viral and cellular membranes. the agent, t-20, which has been approved for hiv inhibition, can restrain gp41 function in the fusion process; nevertheless, it has disadvantages like instability, high cost of production and injection form to be delivered twice a day. methods: several molecules like nb...

Cancer is a leading cause of death globally, claiming about 9.6 million lives and approximately 420 new cases cancer will be diagnosed in the world by year 2025. The aim this study was to synthesize computationally evaluate pharmacological potential some derivatives 9-amino-3-phenylacridone, as topoisomerase II (Topo II) inhibitors. In study, 10 3-phenyl-9-aminoacridone were chemically synthesi...

influenza c virus possesses specific neuraminate-o-acetylesterase as a receptor-destroying function. this enzymatic activity of the viral glycoprotein hef (hemagglutinin, esterase activity and fusion factor) can be visualized in situ by the use of distinct color substrates. hereby the localization, as well as the quantity of synthesized hef protein is detectable. we further developed the estera...

The present study was designed to evaluate the in vitro and silico potential of Schiff bases (Z)-4-ethoxy-N-((5-nitrothiophen-2-yl)methylene)benzenamine (1) (Z)-2,4-diiodo-6-((2-methyl-3-nitrophenylimino)methyl)phenol (2). These were synthesized according a reported method using ethanol as solvent, each reaction monitored on TLC until completion reaction. structures both compounds elucidated sp...

An enzyme hydrolyzing 4to g-carbon y-lactones has been purified 12to 15-fold from rat liver and from human plasma. In rat liver the enzyme is located in the microsomal fraction and may be solubilized by deoxycholate treatment and separated from microsomal B-esterase by ammonium sulfate fractionation. The effects of a series of inhibitors on y-lactonase, B-esterase purified from rat liver micros...