BACKGROUND Adhesion formation after peritoneal surgery is a major cause of postoperative bowel obstruction, infertility, and chronic pelvic pain. In this study, we compared the possible individual effects of phosphatidylcholine (PC), Seprafilm II, and tissue plasminogen activator (t-PA) and the combined effects of phosphatidylcholine and t-PA on postoperative adhesion formation in a rat surgica...

1. Either l-[4,5-(3)H]leucine or [Me-(3)H]choline, or both l-[U-(14)C]leucine and [Me-(3)H]-choline, were injected into the ninth dorsal root ganglion of the frog, and peripheral transport of labelled proteins and/or phospholipids, mostly phosphatidylcholine, was studied by analysis of consecutive segments of the sciatic nerve. 2. At 25 degrees C, approx. 5% of the (3)H-labelled protein was tra...

Single-chain alkylphospholipids, unlike conventional chemotherapeutic drugs, act on cell membranes to induce apoptosis in tumor cells. We tested four different alkylphospholipids, i.e., edelfosine, perifosine, erucylphosphocholine, and compound D-21805, as inducers of apoptosis in the mouse lymphoma cell line S49. We compared their mechanism of cellular entry and their potency to induce apoptos...

Human group-V phospholipase A(2) (hVPLA(2)) is a secretory phospholipase A(2) (PLA(2)) that is involved in eicosanoid formation in such inflammatory cells as macrophages and mast cells. We showed that hVPLA(2) can bind phosphatidylcholine membranes and hydrolyse phosphatidylcholine molecules much more efficiently than human group-IIa PLA(2), which accounts for its high activity on the outer pla...

The principal lung phospholipids were studied following pulse labeling of rabbit lung in vivo with the phospholipid precursors [3H]glycerol and [14C]palmitic acid. Alveolar wash, microsome, and lamellar body fractions subsequently were isolated. The phospholipids and the fatty acid compositions of each phospholipid in each lung fraction were quantified. The appearance of labeled phosphatidylcho...

The gastrointestinal hydrolysis of food proteins has been portrayed in scientific literature to predominantly depend on the activity and specificity proteolytic enzymes. Human bile not considered facilitate proteolysis small intestine, but rather assist intestinal lipolysis. However, human can potentially influence that are largely resistant gastric digestion, which mainly hydrolysed after they...

Silibinin, an active constituent of silymarin possesses several medicinal properties. Although, the poor water solubility and less bioavailability restrict clinical utility silibinin, complex silibinin phosphatidylcholine manifested outstanding biological activities in vivo. Thereby, was prepared with (1:2, w/w) for better therapeutic efficacy their physicochemical properties were analyzed. The...

We present electron density maps (EDMs) of the ripple phase formed by phosphorylcholine lipids such as dimyristoyl phosphatidylcholine (DMPC), palmitoyl-oleoyl phosphatidylcholine (POPC), dihexadecyl phosphatidylcholine, and dilauroyl phosphatidylcholine (DLPC). With the exception of DLPC, the rippled bilayers have a sawtooth shape in all the systems, with one arm being almost twice as long as ...

As part of a population survey and a follow-up study of plasma lipid profiles by high temperature gas-liquid chromatography, we have determined the quantities and relative proportions of all major chemical classes and molecular species of lipids of plasma from 1200 subjects at Visit 2 of the Toronto-McMaster Lipid Research Clinic Prevalence Study. We have compared these values between our 24 su...