variations in phosphatidate phosphohydrolase (pap) activity and triacylglycerol concentration were measured in pregnant and hormone-treated non-pregnant female rats. pap activity in adipose tissue was elevated by 61% during pregnancy. the increase in the enzyme activity was paralleled with a rise in serum triacylglycerol concentration (44%). estradiol injecting into non-pregnant rats increased ...

We examined further the ffects of angiotensin-11 and K+ on phospholipid metabolism in the adrenal zona glomerulosa. In adrenal zona glomerulosa cell preparations, physiological and supraphysiological concentrations of angiotensin-I1 (10"2-10-4 M) and K+ (0-16 m ~ ) elicited doseand time-dependent increases in aldosterone production and (32P)phosphate incorporation into chromatographically purif...

The yeast Pah1p phosphatidate phosphatase, which catalyzes the penultimate step in the synthesis of triacylglycerol and plays a role in the transcriptional regulation of phospholipid synthesis genes, is a cytosolic enzyme that associates with the nuclear/endoplasmic reticulum membrane to catalyze the dephosphorylation of phosphatidate to yield diacylglycerol. Pah1p is phosphorylated on seven (S...

Previous studies have indicated different energy requirements for some platelet responses; these differences could, however, be due to inadequate methodology and differences in platelet preparation. The present study describes the effect of decreasing ATP availability on seven platelet responses measured in gel-filtered human platelets. The cells, prelabelled with 5-hydroxy[3Hitryptamine, 13Hlo...