Differentiating intracellular from extracellular enzyme activity is important in soil enzymology, but not easy. Here, we report on an adjusted sonication method for the separation of intracellular from extracellular phosphatase activity in soil. Under optimal sonication conditions [soil:water ratio  =  1/8 (w/v) and power density  =  15 watt ml(-1)], the activity of alkaline phosphomonoesterase...

A phosphatase, active towards phosphorylase a and phosphorylated proteins casein and histone II-A, was isolated from Saccharomyces cerevisiae 257. The enzyme dephosphorylated glycogen phosphorylase from commercial yeast rendering it inactive. The protein phosphatase activity was not influenced by any metal ions. Phosphorylase phosphatase activity was slightly stimulated by p-nitrophenyl phospha...

Using immunochemical techniques, alkaline phosphatase isoenzymes were determined in tissue samples of breast carcinomas and carcinomas of the gastrointestinal tract. In breast carcinomas only 19% of the patients expressed significant placental alkaline phosphatase activity, compared with 78% in gastrointestinal tumours. The intestinal isoenzyme was found in 50% of the breast carcinomas and in n...

Bile duct ligation causes a five- to sevenfold increase in the activity of rat liver alkaline phosphatase within 12 hours after ligation and a similar rise in the activity of alkaline phosphatase in serum. The increased serum activity is due entirely to the appearance of a new isoenzyme that has the properties of rat liver alkaline phosphatase. The increase in both serum and liver alkaline phos...

Acid phosphatase activity increases above normal in the liver of whole body irradiated animals. It is considered to be one of the adverse radiation effects. Increase in the acid phosphatase activity was dose dependent. Centella asiatica extract, which increased the survival of gamma irradiated Swiss albino mouse when given prior to irradiation was tested to observe changes in the acid phosphata...

This study was aimed to determine the alkaline phosphatase activity following time course (days) in the intestine of broilers. We used a sample of 54 subjects of broilers from hatching period to 56th day of age. Our finding showed a significant increase in the alkaline phosphatase activity at various levels in the different intestinal segments during the first two weeks of life and then decreas...

A partially purified bovine cortical bone acid phosphatase, which shared similar characteristics with a class of acid phosphatase known as tartrate-resistant acid phosphatase, was found to dephosphorylate phosphotyrosine and phosphotyrosyl proteins, with little activity toward other phosphoamino acids or phosphoseryl histones. The pH optimum was about 5.5 with p-nitrophenyl phosphate as substra...