conclusions our successful extraction of purified lps from e. coli isolates of uti patients’ urine samples can be an important step to understand the uti disease conditions. results the silver-stained gel demonstrated both smooth and rough type lps by showing trail-like band patterns with the presence and lacking o-antigen region, respectively. coomassie blue staining showed no band assuring th...

the indian riverine buffaloes are more susceptible to variations in the environment. these variations affect seminal plasma proteins, which in turn affect fertility. this study was conducted to evaluate variations in the indian bubalus bubalis seminal plasma protein profile using two-dimensional polyacrylamide gel electrophoresis (2d-page) during winter and summer seasons, and its relation with...

background: natural products have gained importance recently for the treatment of obesity and its complications, partly because of the side effects of modern drugs.hence, we aimed to study and compare the effect of varying concentrations of momordicacharantiaon adipogenesis and adipolysis using 3t3-l1 pre-adipocyte cell lines. methods: 3t3-l1 pre-adipocytes were procured from the national cente...

Despite numerous attempts to develop and evalute specsc methods for the histochemical demonstration of mucopolysaccharides, definitive information is not readily available. Thls is in spite of the notable contributions of Hale ('46); Steedman ('50); Gomori ('50); Kramer and Windrum ('54); Mowry ('56, '58); Spicer ('60); Belanger and Migicovsky ('61) and Goldstein ('62) to mention only a few. Wi...

A skeletal examination of fetuses is required in regulatory embryo-fetal development studies. This chapter describes a method of skeletal examination using alizarin staining. All fetuses are removed from the mother by caesarean section before birth. The fetuses are first examined externally. For larger species (rabbit and minipig), an internal examination can be performed on the fresh soft tiss...

In developing countries pulmonary tuberculosis is usually diagnosed by detecting acid-fast bacilli (AFB) in sputum using a Ziehl-Neelsen (Z-N) staining method. However, in the field the traditional method of staining is difficult to carry out. This study evaluates the efficiency of two cold staining methods, namely Gabbet’s and a modified two reagent cold staining method compared with the Z-N t...