Regulatory RNAs play important roles in the control of bacterial gene expression. In this study, we investigated gene expression regulation by a putative glycine riboswitch located in the 5'-untranslated region of a sodium:alanine symporter family (SAF) protein gene in the group A Streptococcus pyogenes serotype M49 strain 591. Glycine-dependent gene expression mediated by riboswitch activity w...

Glycine receptors are widely expressed in the mammalian central nervous system, and previous studies have demonstrated that glycine receptors are modulated by endogenous zinc. Zinc is concentrated in synaptic vesicles in several brain regions but is particularly abundant in the hippocampus and olfactory bulb. In the present study, we used patch-clamp electrophysiology of rat hippocampal and olf...

Whole cell voltage-clamp electrophysiology was used to examine interactions between GABA and glycine at inhibitory amino acid receptors on rat olfactory bulb neurons in primary culture. Membrane currents evoked by GABA and glycine were selectively inhibited by low concentrations of bicuculline and strychnine, respectively, suggesting that they activate pharmacologically distinct receptors. Howe...

Although all bipolar cells in the retina probably use the excitatory transmitter glutamate, approximately half of the cone bipolar cells also contain elevated levels of the inhibitory transmitter glycine. Some types of cone bipolar cells make heterologous gap junctions with rod amacrine cells, which contain elevated levels of glycine, leading to the hypothesis that the bipolar cells obtain thei...

High-affinity glycine transport in neurons and glial cells is a primary means of inactivating synaptic glycine. We have synthesized a potent selective inhibitor of glycine transporter 1 (GlyT1), and characterized its activity using a quail fibroblast cell line (QT6). The glycine transporters GlyT1A, GlyT1B, GlyT1C, and GlyT2 were stably expressed in QT6 cells. The transporters expressed in thes...

Abstract In this study, we presented two reliable methods, the sol–gel and autocombustion, to synthesis a high purity BiFeO 3 single phase with low calcination time using glycine as chelating agent. The glycine-autocombustion method produced either or concentrations of reactants (Bi(NO ) + Fe(NO ), while glycine-sol–gel delivered reactants. case tartaric acid urea agents, mixtures Bi 25 FeO 40 ...

Abstract The hydrothermal liquefaction (HTL) of biomass is a strategic process to convert wet and waste feedstocks into liquid biofuel. In this work, we investigated the glucose glycine, alone together, mimic composition low-lipid content biomass. Experimental tests were performed in batch setup temperature range 200–350 °C. As feeding changed, distribution among different phases (gas, solid, b...

4-Phenyl-3-butenoic acid (PBA) has been shown in vitro to be a turnover-dependent inactivator of peptidylglycine alpha-monooxygenase (PAM), the rate-limiting enzyme involved in the formation of amidated neuropeptides from their glycine-extended precursors. In the studies reported herein, we have shown that PBA produces a dose-dependent (50-500 mg/kg s.c.) inhibition of serum PAM activity in nor...

drought, as an abiotic stress inflicts destructive and harmful effects on plants at their different growth stages. plants react differently to drought. among their reactions is the production of such osmolytes as proline and glycine betaine. it seems that, through exogenous application of these materials, plant resistance to drought stress can be increased. therefore, a factorial experiment, ba...