AIMS Angiotensin-converting enzyme (ACE), which metabolizes many peptides and plays a key role in blood pressure regulation and vascular remodeling, is expressed as a type-1 membrane glycoprotein on the surface of different cells, including endothelial cells of the heart. We hypothesized that the local conformation and, therefore, the properties of heart ACE could differ from lung ACE due to di...

One of the primary challenges in making sense the dramatic increase in human genotype data is finding suitable phenotype information for correlational analyses. While the price of genotyping has fallen dramatically and promises to continue to decrease, the cost of generating the phenotypes necessary to take advantage of this data has held steady or even increased. Until recently, human phenotyp...

With large volumes of health care data comes the research area of computational phenotyping, making use of techniques such as machine learning to describe illnesses and other clinical concepts from the data itself. The “traditional” approach of using supervised learning relies on a domain expert, and has two main limitations: requiring skilled humans to supply correct labels limits its scalabil...

2 Introduction This document demonstrates the functionality of the flowType package for phenotyping FCM assays. flowType uses a simple threshold, Kmeans, flowMeans or flowClust to partition every channel to a positive and a negative cell population. These partitions are then combined to generate a set of multi-dimensional phenotypes. For more details on how this package can be used in a complet...

Most gene mutations and biologically active molecules cause complex responses in animals that cannot be predicted by cell culture models. Yet animal studies remain too slow and their analyses are often limited to only a few readouts. Here we demonstrate high-throughput optical projection tomography with micrometre resolution and hyperdimensional screening of entire vertebrates in tens of second...