Leptin biosynthesis in adipose cells in vivo is increased by food intake and decreased by food deprivation. However, the mechanism that couples leptin production to food intake remains unknown. We found that addition of leucine to isolated rat adipocytes significantly increased leptin production by these cells, suggesting that postprandial leptin levels may be directly regulated by dietary leuc...

BACKGROUND Pseudomonas putida KT2442 is a natural producer of polyhydroxyalkanoates (PHAs), which can substitute petroleum-based non-renewable plastics and form the basis for the production of tailor-made biopolymers. However, despite the substantial body of work on PHA production by P. putida strains, it is not yet clear how the bacterium re-arranges its whole metabolism when it senses the lim...

Introduction and Aim: Tuberculosis (TB) remains a major public health concern with its high incidence mortality. In developing countries, smear microscopy continues to be the primary diagnostic tool for diagnosis of TB. Sputum samples processing under resource-limited settings could hazardous, which urges safe efficient techniques. This study aimed evaluate efficacy two different pot methods: P...

Medium-chain-length polyhydroxyalkanoates (mcl-PHAs), polyesters produced by bacterial fermentation of sugars and/or lipids, are potential bioplastic alternatives to petroleum-derived plastics. Lignocellulose is an abundant renewable source for fermentation. However, chemical or enzymatic release the requires a pretreatment step dispel rigid structure lignocellulose and enhance sugar access. Mo...

Pseudomonas resinovorans produced poly-beta-hydroxyalkanoates (PHAs) when grown on hydrocarbons but not on glucose. In a chemostat culture, the PHA composition was beta-hydroxybutyrate (C4)-beta-hydroxyhexanoate (C6)-beta-hydroxyoctanoate (C8)-beta-hydroxydecanoate (C10) (1:15:75:9) on octanoate and C4-C6-C8-C10 (8:62:23:7) on hexanoic acid. Contrary to the reported behavior of Pseudomonas oleo...

The potential of bio based plastics for replacement of petrochemical plastics is 90%, corresponding to 240 million tons per year.1 Bio-based materials such as polynucleotides, polyamides, polysaccharides, polyoxoesters, polythioesters, polyanhydrides, polyisoprenoids and polyphenols are potential candidates for substitution of synthetic plastics.2 Polyhydroxyalkanoates (PHAs), belonging the gro...