The aim of this study was to find a proper method for improvement of ischemic condition in the rat hind limb and also to observe the efficacy of cell engraftment with alginate/gelatin three-dimensional scaffolds. Eighteen male Wistar rats weighing 200 to 250 g were randomly divided into three groups (n = 6) including a) ischemia group; in which femoral artery was removed after ligation at the d...

BACKGROUND Increased blood and plasma viscosity, hematocrit, fibrinogen, and activation of coagulation and leukocytes have been reported in patients with claudication; however, their associations with symptomatic and asymptomatic peripheral arterial disease have not been reported in an epidemiological study. METHODS AND RESULTS Blood and plasma viscosity, hematocrit, fibrinogen, urinary fibri...

Purpose: To determine the usefulness of pulse oximetry in patients receiving iloprost infusion for treatment of critical limb ischemia unsuitable for revascularization. Material and methods: A total of 42 patients [28 male, mean age 61.80±5.81 years] were included. Patients were those who considered as unsuitable for revascularization based on angiographic study and received single-dose infusio...

Recently, epidemiological studies on the etiology of peripheral neuropathies have revealed that hyperlipidemia is a novel risk factor. Plasma lipid levels were confirmed to be associated with the incidence of many peripheral neuropathies including axonal distal polyneuropathy, vision and hearing loss, motor nerve system lesions and sympathetic nerve system dysfunction. Moreover, different lipid...

Peripheral Recording To monitor spinal cord function, peripheral input/output relationships _must be analyzed. Peripheral nerves are, therefore, important sites for stimulation and recording. As is typical for EPs, selection of electrodes and their use is essentially the same for stimulation and recording. There are several types of electrodes used for peripheral nerve stimulation or recording;...

Vasodilation of lower leg arterioles is impaired in animal models of chronic peripheral ischemia. In addition to arterioles, feed arteries are a critical component of the vascular resistance network, accounting for as much as 50% of the pressure drop across the arterial circulation. Despite the critical importance of feed arteries in blood flow control, the impact of ischemia on feed artery vas...

Nitric oxide (NO) plays a complex role in the pathophysiology of cerebral ischemia. In this study, mutant mice with disrupted type I (neuronal) NO synthase (nNOS) were compared with wild-type littermates after permanent focal ischemia. Cerebral blood flow in the central and peripheral zones of the ischemic distribution were measured with laser doppler flowmetry. Simultaneously, microdialysis el...

Nicotinamide phosphoribosyltransferase (NAMPT) is an important neuroprotective factor in cerebral ischemia, and it has been reported that NAMPT inhibitors can aggravate neuronal injury in the acute phase. However, because it is a cytokine, NAMPT participates in many inflammatory diseases in the peripheral system, and its inhibitors have therapeutic effects. Following cerebral ischemia, the peri...

BACKGROUND Reperfusion aggravates nerve ischemic fiber degeneration, likely by the generation of reduced oxygen species. We therefore evaluated if racemic alpha-lipoic acid (LA), a potent antioxidant, will protect peripheral nerve from reperfusion injury, using our established model of ischemia-reperfusion injury. METHODS We used male SD rats, 300+/-5 g. Ischemia was produced by the ligature ...

Patients presenting with chief complaint of chest pain or other signs suggestive of acute coronary syndrome (ACS) in hospital is often time-consuming, expensive and problematic to arrive to the definitive diagnosis for the cause of chest pain. Recent research has found that ischemia-modified albumin (IscMA) is an ideal biomarker for ischemia. It is highly sensitive and detectable in the early p...