carotenoids are red, orange and yellow pigments, which are widely distributed in fruit and vegetables. the aim of this research is to investigate the effect of conventional, high pressure and microwave cooking on the concentrations of β-carotene in carrot and sweet corn. raw carrots were washed, peeled and diced to a uniform size and then frozen at -70°c. sweet corn was also provided in a simil...

We have developed a simplified and improved high performance liquid chromatography (HPLC) method for the detection and quantitation of tissue lipid using a new laser light-scattering detector (Varex model ELSD II). This detector has a limit of sensitivity of 50 ng for neutral lipid and 200 ng for most phospholipids with excellent reproducibility. By coupling the ELSD II with a ternary gradient ...

A mutant of Mycobacterium smegmatis defective in mycolic acid biosynthesis was isolated following chemical mutagenesis. Fatty acids were extracted from the mutant and subjected to structural analysis by thin-layer chromatography and high-performance liquid chromatography (HPLC) of both methyl and p-bromophenacyl ester derivatives. Thin-layer chromatography did not show the presence of any fatty...

Chloroquine and Hydroxychloroquine drugs are widely prescribed for malaria disease. Since the end of 2019, humans have been under threat due to a disease called (COVID-19), which was first reported in China. Many methodical approaches quantify chloroquine hydroxychloroquine blood, urine, plasma, serum, pharmaceutical dosage form. Some these techniques spectrophotometry, liquid chromatography wi...

To quantify the colchicine (alkaloid) present in Gloriosa superba by High Performance Liquid Chromatography (HPLC) method. Gloriosa superba species are known for their content of colchicine, a valuable alkaloid in the seeds and tubers (corms). HPLC analysis was carried out to confirm the presence of colchicine in Gloriosa superba. Treatment of gibberellic acid and Psedomonas aerugenosa improved...

T he past several years have seen a significant resurgence of interest in cap i l l a ry electro ch ro m at ography(CEC), a hybrid technique that combines the high efficiency owing to the flow characteristics related to electroosmosis with the selectivity of high performance liquid chromatography(HPLC). In CEC, separations are generally carried out using fused silica capillaries packed with HPL...

A improved, simple and economic reverse phase high performance liquid chromatography (RP-HPLC) method has been developed and validated as per ICH guidelines for the quantification of acetic acid present in Gefitinib as an solvent impurity. Validation studies demonstrated that the proposed RP-HPLC method is simple, specific, rapid, reliable and reproducible. The high recovery and low relative st...

Two unknown impurities were detected in verapamil hydrochloride bulk drug using isocratic reversed-phase high performance liquid chromatography (HPLC). These impurities were isolated by preparative HPLC. Spectral data for the isolated impurities were collected. Based on the spectral data derived from two-dimensional nuclear magnetic resonance (2D-NMR) spectroscopy and mass spectrometry (MS), im...

high-performance liquid chromatography (hplc) methods employing ultraviolet (uv) detector are not sufficiently sensitive to measure the low plasma concentrations following single oral dose of imipramine. therefore, in the present study a simple, rapid and yet sensitive hplc method with uv detection was developed and validated for quantitation of imipramine in human plasma samples. an efficient ...

A newly developed single quad mass spectrometry (MS) detector was coupled to a ultra-high performance liquid chromatography (UPLC) system and implemented in the routine quality control (QC) and impurity analysis of four therapeutic peptides, namely bleomycin sulfate, tyrothricin, vancomycin HCl and bacitracin, which were selected given their multi-component drug nature and their closely structu...