Sampling was done using 90 post larvae which were produced by reproduction of some broodstocks of Fenneropenaeus indicus in one day and reared in the same situation for 4 months. Samples were divided into 3 groups: high, medium and low growth (based on weight and length). Genomic DNA was extracted from muscle tissue using the phenol-chloroform method. The polymerase chain reaction (PCR) was car...

BACKGROUND Vulvovaginal candidiasis is a common infection. The aim of this study was to identify the species of vaginal Candida isolates by using multiplex PCR technique. METHODS 191 isolates from patients admitted to Mahdieh hospital were identified. The vaginal swab specimens were cultured on Sabouraud Dextrose Agar. The ITS1 region between the 18S and 5.8S rRNA genes and a specific DNA fra...

BACKGROUND To determine the epidemiological status of cutaneous leishmaniasis outbreak, isolation and identification of the agent parasite, Leishmania, using PCR method in Gonbad-e Qabus County, north Iran, during 2006-2007. METHODS Data were collected on the prevalence of scars and ulcers over a period of 3 months among 6990 inhabitants of five villages around Gonbad-e Qabus County, north Ir...

A fluorescence-based method for polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) analysis, F-SSCP, was developed in which the target sequence is amplified by the PCR using fluorescent primers. The amplified products are then heat-denatured and applied to a water-jacket controlled gel in an automated DNA sequencer. The separated strands are detected as laser-excited f...

Hydropericardium syndrome (HPS) agent was purified by sucrose gradient centrifugation. The polypeptides of HPS agent were separated through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and identified by known protein markers. Eight polypeptides having molecular weights ranging from 119.0 to 15.7 KDa were observed. The nucleic acid of HPS agent was extracted and determin...

Gel electrophoresis technique is an indispensable tool in biotechnology and among other related fields for separation of nucleic acids proteins. This study determined the potential selected cassava sweet potato starch DNA as alternative to agarose gel. The sample pH, gelling temperature time were by Light transmittance method proposed Craig et al. [1] Standard procedure was used gel electrophor...