We present a protocol for effi cient amplifi cation of a large number of DNA targets using a single-tube polymerase chain reaction (PCR) based on PCR suppression. This method allows amplifi cation of each DNA target with only one target-specifi c primer whereas the other primer is common for all targets. Thus, this approach requires n+1 primers for n targets instead of 2n in conventional PCR an...

Seed vigor is an important trait for tobacco production. However, the evaluation of seed using molecular biomarkers scarcely reported in tobacco. In this study, development marker isopropylmalate synthase NtIPMS was conducted to detect ageing degree and priming effect Quantitative real-time PCR (qRT-PCR) analysis showed that expression significantly induced at initial imbibition stage during ge...

The purpose of this article was to identify the diagnostic significance HPV testing and cytoscopic examination in terms cervical pathology. In course study during 2020-2021. 10,716 women aged 25-65 years were examined, who did not present complaints, indicating gynecological pathology have a history erosive lesions cervix. study, sensitivity, specificity PCR test calculated detecting screening ...

Allozyme and PCR-based molecular markers have been widely used to investigate genetic diversity and population genetic structure in autotetraploid species. However, an empirical but inaccurate approach was often used to infer marker genotype from the pattern and intensity of gel bands. Obviously, this introduces serious errors in prediction of the marker genotypes and severely biases the data a...

Abstract Background Polymerase chain reaction (PCR) based SARS-CoV-2 RNA detection and serological antibody tests give a proof of Coronavirus Disease 2019 (COVID-19) infection. Several variables can influence the consequences these tests. Inflammatory markers among mild severe patients COVID-19 showed dissimilarity in inflammatory while computed tomography (CT) infected with used to evaluate in...

BACKGROUND Triple-negative breast cancer (TNBC) attracts a disproportionate share of intensive research because of its poor prognosis. Standard anthracycline- and taxane-based regimens still yield an unsatisfactorily low rate of pathologic complete response (pCR). The pCR rate is a recognized surrogate marker for good long-term survival. METHODS A multicentric, retrospective study was conduct...

Amplified fragment length polymorphism (AFLP) DNA fingerprinting is a firmly established molecular marker technique, with broad applications in population genetics, shallow phylogenetics, linkage mapping, parentage analyses, and single-locus PCR marker development. Technical advances have presented new opportunities for data analysis, and recent studies have addressed specific areas of the AFLP...

the purpose of this study was to identify the rate of polymorphism in bm6444, inra13 and orahh35 microsatellite markers associated with inhibin gene in sanjabi sheep. for this purpose, blood samples were taken randomly from 100 sanjabi sheep (78 females and 22 males) at mehregan breeding station at kermanshah. modified salting out method was employed for extracting dna. subsequently, polymerase...