Abstract Thermal profiling provides the understanding needed to enhance reliability of Polymerase Chain Reaction (PCR) systems, but is difficult perform experimentally when reagents are housed in capillary tubes. The use 3D numerical simulation with COMSOL here showed that tubes attached a slider moved over 3 differentially heated blocks without slots, central axis temperature points tube provi...

The aim of this study was to choose the optimal variant of PCR examination of faeces to detect Echinococcus multilocularis infection which would allow to reduce the influence of different inhibitors in faeces. The investigation was carried out by comparison of 3 different methods of DNA isolation from faeces and different DNA dilutions used in PCR. Thirty five intestines of red foxes were used....