Recombinant HIV-Tat (Tat) induces extensive apoptosis in peripheral blood mononuclear cells (PBMCs) cultured in typical CO2 incubators, which are equilibrated with air (21% O2). However, as we show here, Tat apoptosis induction fails in PBMCs cultured at physiological oxygen levels (5% O2). Under these conditions, Tat induces PBMCs to divide, efficiently primes them for HIV infection, and suppo...

The effects of moxifloxacin, a new methoxyfluoroquinolone, on the production of proinflammatory cytokines from human peripheral blood mononuclear cells (PBMCs) were evaluated. Moxifloxacin inhibited the production of tumor necrosis factor alpha (TNF-alpha) and/or interleukin-6 (IL-6) by PBMCs stimulated with lipopolysaccharide (LPS), lipoteichoic acid (LTA), and heat-killed bacteria in a concen...

BACKGROUND Telomerase activity is closely associated with the expression of human telomerase reverse transcriptase (hTERT) mRNA; although it can be induced in hepatocytes during liver regeneration, its dynamic change in patients with liver failure has remained unclear. OBJECTIVES We investigated the variation and significance of hTERT mRNA expression in peripheral blood mononuclear cells (PBM...

Objective(s):Immunosuppressive property of mesenchymal stem cells (MSCs) has great attraction in regenerative medicine especially when dealing with tissue damage involving immune reactions. The most attractive tissue sources of human MSCs used in clinical applications are bone marrow (BM), adipose tissue (AT), and Wharton's jelly (WJ) of human umbilical cord. The current study has compared immu...

HTLV-1 is the causative agent for a neurologic disease named HTLV-I- associated myelopathy/tropical spastic paraparesis (HAM/TSP). Paraparesis of the lower limbs which appears gradually is the most common clinical feature of this disease. The indirect involvement of the nervous system by lymphocytes is more probable than the direct attack of the virus to the neurons. The proviral load (PVL) is ...

Human peripheral blood mononuclear cells (PBMCs) are the key drivers of the immune responses. These cells undergo activation, proliferation and differentiation into various subsets. During these processes they initiate metabolic reprogramming, which is coordinated by specific gene and protein activities. PBMCs as a model system have been widely used to study metabolic and autoimmune diseases. H...

Expression profiling has demonstrated that transcriptomes of primary malignancies differ from those in normal tissue. It is unknown, however, whether there exist “surrogate” transcriptional markers in peripheral blood mononuclear cells (PBMCs) of patients with solid tumors. We identified transcripts expressed differentially between PBMCs from renal cell carcinoma patients and normal subjects, s...

Background & Aims: Verapamil as a calcium channel blocker has been broadly used in the treatment of many cardiovascular diseases such as hypertension and arrhythmia. Furthermore, the anti-tumor/ antiinflammatory effects of verapamil have been shown. In the present study, the cytotoxic effect of verapamil on human peripheral blood mononuclear cells (PBMCs) and BALB/c peritoneal macrophges has be...

Abstract Background Factors predicting peripheral blood total HIV-1 DNA size in chronically infected patients with successfully suppressed viremia remain unclear. Prognostic power of such factors are clinical significance for making decisions. Methods Two sets study populations were included: 490 China AIDS Clinical Trial (CACT) participants (Training cohort, followed up 144 to 288 weeks) and 1...

objective(s):immunosuppressive property of mesenchymal stem cells (mscs) has great attraction in regenerative medicine especially when dealing with tissue damage involving immune reactions. the most attractive tissue sources of human mscs used in clinical applications are bone marrow (bm), adipose tissue (at), and wharton's jelly (wj) of human umbilical cord. the current study has compared...