The Parapoxvirus Orf virus represents a promising candidate for novel vector vaccines due to its immune modulating properties even in nonpermissive hosts such as mouse or rat. The highly attenuated Orf virus strain D1701 was used to generate a recombinant virus (D1701-VrVp40) expressing nucleoprotein p40 of Borna disease virus, which represents a major antigen for the induction of a Borna disea...

Ecthyma contagiosum is a viral disease of sheep and goats, which is caused by Parapoxvirus. Primary cell cultures of lamb testis, lamb kidney, rabbit kidney and permanent cell lines: foetal sheep kidney (FSK), sheep foetal thymus (SFT), sheep foetal muscle (SFM), sheep plexus choroideus (SPC), bovine trachea (BTR), embryonic bovine trachea (EBTR), Madin Darby bovine kidney (MDBK) and baby hamst...

A high-throughput multiplexed assay was developed for the differential laboratory detection of foot-and-mouth disease virus (FMDV) from viruses that cause clinically similar diseases of livestock. This assay simultaneously screens for five RNA and two DNA viruses by using multiplexed reverse transcription-PCR (mRT-PCR) amplification coupled with a microsphere hybridization array and flow-cytome...

BACKGROUND The Orf virus (ORFV) is the causative agent of orf, a globally-occurring, acute, pustular, contagious disease affecting sheep, goats and humans with a worldwide distribution. Currently, the genomic analysis of four ORFV strains from the Fujian province in southern China and a NA1/11 strain isolated from the Jilin province in northeast China have been reported. However, little is know...

1. Kroon EG, Mota BE, Abrahão JS, da Fonseca FG, de Souza Trindade G. Zoonotic Brazilian vaccinia virus: from field to therapy. Antiviral Res. 2011;92:150–63. http://dx.doi.org/10.1016/ j.antiviral.2011.08.018 2. Assis FL, Borges IA, Mesquita VS, Ferreira PC, Trindade GS, Kroon EG, et al. Vaccinia virus in household environment during bovine vaccinia outbreak, Brazil. Emerg Infect Dis. 2013;19:...

In western Canada, Eastern Grey Squirrels (Sciurus carolinensis) were introduced to Stanley Park in the city of Vancouver, British Columbia, shortly before 1914. The three to four pairs of introduced Eastern Grey Squirrels came from eastern Canada (Robinson and Cowan 1954). In the early 1980s, Eastern Grey Squirrels colonized the rest of the city of Vancouver, including many of its urban parks....

The dsRNA binding protein (RBP) encoding gene of parapoxviruses (PPVs) from the Dromedary camels, inhabitating different geographical region of Rajasthan, India were amplified by polymerase chain reaction using the primers of pseudocowpoxvirus (PCPV) from Finnish reindeer and cloned into pGEM-T for sequence analysis. Analysis of RBP encoding gene revealed that PPV DNA from Bikaner shared 98.3% ...

Borges AS, et al. Araçatuba virus: a vac-cinialike virus associated with infection in humans and cattle. from humans and cattle in Rio de Ja-neiro State: Cantagalo virus may derive from Brazilian smallpox vaccine. Virol-Ferreira F, et al. Natural human infections with vaccinia virus during bovine vaccinia outbreaks.proved assay to detect neutralizing-anti-body following vaccination with diluted...

markable. Laboratory evaluations were significant for a leukocyte count of 1,500/mm (normal value, 4,500–11,000/mm) and macrocytic anemia (hematocrit, 30%; hemoglobin level, 9.8 g/dL; mean corpuscular volume, 104.6 fL). The absolute neutrophil and lymphocyte counts were 870 and 600/mm, respectively. The blood urea nitrogen level was 34 mg/dL (normal value, 7–22 mg/dL), and the creatinine level ...