AIM/BACKGROUND Conflicting results have been reported concerning the prognostic value of DNA flow cytometric variables (DNA ploidy, DNA index, %S phase fraction) in breast cancer. Selection bias and differences in treatment may have contributed to these conflicting prognostic results. Differences in tissue processing, the number of nuclei measured, DNA histogram/cell cycle analysis, and intra-t...

OBJECTIVE Follicular lymphoma (FL) is one of the most common lymphomas, and is characterized by t(14;18)(q32;q21) in more than 80% of patients. The aim of this study was to determine the rate of t(14;18) positivity based onthe detection of mbr or mcr in paraffin-embedded tissue samples. MATERIAL AND METHODS The study included 32 paraffin-embedded tissue samples collected from 32 consecutive F...

Background: Cytoplasmic lipid droplets are important in cancer metabolism and a clear relationship has been established between their accumulation and increased tumour grade in glioma. The development of the novel immunohistochemical marker adipophilin has proven to be a useful method of detecting lipid droplets in paraffin embedded tissue from many diseases. Our aim was to assess the distribut...

A comprehensive mutation detection assay is described for the entire coding region and all splice site junctions of TP53. The assay is based on denaturing gradient gel electrophoresis, which follows either multiplex polymerase chain reaction (PCR) applied to DNA extracted from fresh or frozen tissue samples or nested PCR applied to DNA extracted from paraffin-embedded tissue samples. In both in...

With both a classic DNA preparation protocol (including removal of paraffin wax and protein digestion) and a DNA extraction protocol with Chelex 100, the hepatitis B virus genome was searched for using the polymerase chain reaction (PCR) in 30 samples of paraffin wax embedded liver tissue from patients with chronic hepatitis. The classic protocol was more sensitive than the rapid Chelex 100 pro...

Heat-induced epitope retrieval (HIER) is widely used for immunohistochemistry on formalin fixed paraffin-embedded tissue and includes temperatures well above the melting point of paraffin. We therefore tested whether traditional xylene-based removal of paraffin is required on sections from paraffin-embedded tissue, when HIER is performed by vigorous boiling in 10 mM Tris/0.5 mM EGTA-buffer (pH=...

Introduction: Oral Lichen Planus (OLP) is one of the most common mucocutaneous lesion, and its etiologic factors have not been elucidated yet. OLP has two different clinical forms including reticular and erosive. Mast cells function in non-specific immunology is an etiologic factor involved in the formation of lichen planus. This study aimed at determining the pattern of mast cells presence in ...

Fresh surgical specimens and blocks, as listed in Table I, were taken from 60 cases, males and females, ranging in age from 22 to 70 years. Fresh surgical specimens were obtained from St. James' Hospital, London (Dr. G. T. Allen, Mr. N. C. Tanner and Mr. A. M. Desmond). Surgical specimens fixed in formalin for variable lengths of time were collected from St. Mark's Hospital, London (Dr. B. Mors...

Background and Aims: Retinoblastoma tumors are the most common intraocular malignancy in childhood, leading to death after two years. The Human Adenovirus (HAdV) infection could be critical in the retinoblastoma pathogenesis due to the virus and retinoblastoma 1 interactions. The objective of the current study was to investigate the possible presence of the HAdV genome in the retinoblastoma pat...