The interaction of biotinylated G4 poly (amidoamine) (PAMAM) dendrimer conjugates and G4 PAMAM dendrimers with in vitro models of the blood brain barrier (BBB) was evaluated using Langmuir Blodgett monolayer techniques, atomic force microscopy (AFM) and lactate dehydrogenase measures of cell membrane toxicity. Results indicate that both G4 and G4 biotinylated PAMAM dendrimers disrupt the compos...

Increasing the energy density in electric double-layer capacitors (EDLCs), also known as supercapacitors, remains an active area of research. Specifically, there is a need to design and discover electrode electrolyte materials with enhanced electrochemical storage capacity. Here, using fully atomistic molecular dynamics (MD) simulations, we investigate performance hyper-branched ``poly(amidoami...

Poly(amidoamine) (PAMAM) dendrimers of different generations with carboxyl, acetyl, and hydroxyl terminal groups and a folic acid (FA)-dendrimer conjugate were separated and analyzed using reverse-phase high performance liquid chromatography (HPLC). Analysis of both the individual PAMAM derivatives and the separation of mixed generations can be achieved using a linear gradient 0-50% acetonitril...

An Affibody based dual imaging probe (PET and optical imaging) has been successfully developed. Dendrimer PAMAM G0 was used as a platform to assemble an NIRF dye, a metal chelator, and Affibody for dual modality imaging of ovarian cancer. Excellent tumor imaging quality was achieved in both modalities in the living tumor mice models.

H NMR spectra to support data for Table 1 and Figure 1: For synthesis of hydrophobically modified dendrimers, dodecyl chloroformate was adjusted to 5, 10 and 20 mol % of G5OH PAMAM dendrimer hydroxyl groups, and the amount of cholesteryl chloroformate was 0.86, 2, 5, and 20 mol % of dendrimer hydroxyl groups. The corresponding modified dendrimers were designated as D1, D2, D3 and C1, C2, C3, C4...

Gene therapy offers a promising cure of brain glioma and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is able to induce cell apoptosis of glioma selectively without affecting the normal cells. In this study, the nanoscopic high-branching dendrimer, polyamidoamine (PAMAM), was selected as the principal vector. Angiopep-2, which can target to the low-density lipoprotein recepto...

Experimental data indicates that poly(amidoamine) (PAMAM) dendrimers flatten when in contact with a substrate, i.e. they are no longer spherical, but resemble flat disks. In order to better understand the deformation behavior of these branched polymers, a series of atomistic molecular dynamics simulations is performed. The resulting flattened dendrimer conformations are compared to atomic force...

A target-specific MRI contrast agent for tumor cells expressing high affinity folate receptor was synthesized using generation five (G5) ofpolyamidoamine (PAMAM) dendrimer. Surface modified dendrimer was functionalized for targeting with folic acid (FA) and the remaining terminal primary amines of the dendrimer were conjugated with the bifunctional NCS-DOTA chelator that forms stable complexes ...

Recognition and binding of anions in water is difficult due to the ability of water molecules to form strong hydrogen bonds and to solvate the anions. The complexation of two different carboxylates with 1-(4-carbomethoxypyrrolidone)-terminated PAMAM dendrimers was studied in aqueous solution using NMR and ITC binding models. Sodium 2-naphthoate and sodium 3-hydroxy-2-naphthoate were chosen as c...

Multivalent binding, the simultaneous binding of multiple ligands to multiple receptors, has played a central role in a number of pathological processes, including the attachment of viral, parasitic, mycoplasmal, and bacterial pathogens. These biological activities have been extensively investigated to promote targeting of specific cell types, and biological multivalent inhibitors have yielded ...