Coxiella burnetii, the etiological agent of Q Fever, is a zoonotic pathogen distributed worldwide. It has been reported that virulent strains of C. burnetii are poorly internalized by monocytes compared to avirulent variants. Virulence is also associated to the formation of pseudopodal extensions and transient reorganization of filamentous actin. In this article, we investigated the ability of ...

Background: In vitro maturation (IVM) is a promising treatment option for certain infertile women. Nowadays, with the aid of the Polscope, it has become possible to evaluate zona pellucida (ZP) and spindle as parameters of oocyte quality. The goal was to investigate the relationship between the presence of the meiotic spindle and ZP birefringence with morphology of the in vivo and in vitro matu...

PURPOSE Currently, no studies exist, which attest the suitability of the ovine intervertebral disc as a biomechanical in vivo model for preclinical tests of new therapeutic strategies of the human disc. By measuring the intradiscal pressure in vivo, the current study attempts to characterize an essential biomechanical parameter to provide a more comprehensive physiological understanding of the ...

in order to do in vitro fertilization (ivf), 435 oocytes were collected from 20 super ovulated nmri female mice, in m2 media with 4% bsa. 85% (n=370) of collected oocytes were in mii phase. epididymal sperms were added to 332 oocytes in t6 media drops with 4% bsa and incubated. fertilization rate was 79.8% (n=265) out of which 27.5% (n=73) grew to morula and 20.8% (n=55) achieved blastocyst sta...

background:   objective: in this study, we supplemented a chemically defined medium with different combinations of selenium, calcium and calcium ionophore concentrations to obtain the best rate of human oocytes maturation, survival, and fertilization.   materials and methods: as an experimental study, three combinations of [selenium (5 μg/ml), calcium (5 μg/ml) and calcium ionophore (1 μg/ml)],...

rate of in vitro maturation of oocytes is one of the challenges of assisted reproductive techniques. in this study we investigated the effects of supplementation of cysteamine on the rate of in vitro maturation of oocytes in two different media. germinal vesicle oocytes were collected from mouse ovary and cultured in two media (tcm199 and meme) with 0, 50, 100, 200, 500 µm/ml cysteamine. number...

Abstract Ovine respiratory complex is a significant cause of death in sheep flocks, where Pasteurella multocida the most frequent microorganisms isolated from animals with pneumonia. There an urgent need to refine use different antimicrobials avoid problem antimicrobial resistance and optimize control this disease ovine livestock. The first step approaching gaining insight into susceptibility p...

ABSTRACT This study aimed to evaluate sweet potato feed as an alternative energy supplement for ovine. A latin-square design was used assess the effects of feeding animals 0, 0.5, 1 and 1.5% their body weight in well ryegrass hay (Lolium multiflorum) twice daily, mineral salt water ad libitum. During this 8 males were housed metabolic cages with urine feces collectors. Each round experimentatio...

Background: The present study was carried out to investigate how the interactions between aging, vitrification, and post-warming interval affect the credibility of sheep MII-oocytes for in vitro fertilization (IVF), intracytoplasmic injection (ICSI), and parthenogenetic activation (PA). Materials and Methods: The vitrified-warmed oocytes, either immediately (immediate group: IG) or two hours po...

Background: Before human MII oocytes are vitrified they are usually denuded from their cumulus cells. In this study we wanted to investigate the effects of an intact corona radiata on the vitrification and fertilization of human oocytes. Materials and Methods: The study comprised two different parts. In Part 1, 36 MII stage oocytes, from 6 patients, were randomly assigned into a control group, ...