BACKGROUND Organophosphates (OPs) are neurotoxic compounds for which current methods of elimination are unsatisfactory; thus bio-remediation is considered as a promising alternative. Here we provide the structural and enzymatic characterization of the recently identified enzyme isolated from Pseudomonas pseudoalcaligenes dubbed OPHC2. OPHC2 belongs to the metallo-β-lactamase superfamily and exh...

It is shown from the kinetics of inhibition of cholinesterase by N-methylcarbamates and organo-phosphates that the LD50 dose is likely to be a much greater multiple of the dose causing signs of poisoning in 50%-of the animals (the ED50) for the carbamates than for the organophosphates. The expected difference was demonstrated by a comparison of the LD50s and ED50s, intravenous and intramuscular...

An overview of general strategy, standard procedures, and critical points, which may be found during carrying out an OPCW Proficiency Test concerning detection and identification of scheduled compounds relevant to Chemical Weapon Convention, has been presented. The observations have been illustrated following the case of the Eight OPCW Designated Laboratories Proficiency Test, which was perform...

It has been shown that several hydrolytic enzymes with different specificities undergo similar reactions with diisopropyl phosphorofluoridate (1) and other related toxic organophosphorus compounds (2). These conclusions were based on kinetic studies, particularly the influence of pH on the rates of reaction, and on inhibition characteristics. Reactions of this type have provided a unique method...

1 Associate Professor, Department of Biochemistry, R.C.S.M. Govt. Medical College, Kolhapur 2 Technician, Department of Biochemistry, Govt. Medical College, Miraj 3 Ph D student, Department of Biochemistry, Govt. Medical College, Miraj Corresponding Author Dr Karan Ningappa Pujari Associate professor, Department of Biochemistry, R.C.S.M. Govt. Medical College, Kolhapur (Maharashtra, India). Ema...

A promoter that enabled high-level expression of the target gene during the stationary phase in the absence of an inducer would facilitate the efficient production of heterogeneous proteins at a low cost. In this study, a genome-scale microarray-based approach was employed to identify promoters that induced high-level expression of the target genes in Bacillus subtilis from the late log phase t...

Polyvinyl chloride (PVC) can be used to develop reaction beaker which acts as electrochemical cell for the measurement of OP pesticides. Being chemically inert, corrosion resistant, and easy in molding to various shapes and size, PVC can be used for the immobilization of enzyme. Organophosphorus hydrolase was immobilized covalently onto the chemically activated inner surface of PVC beaker by us...

A method was developed to monitor dynamic changes in protein structure and interfacial behavior on surfaces by single-molecule Förster resonance energy transfer. This method entails the incorporation of unnatural amino acids to site-specifically label proteins with single-molecule Förster resonance energy transfer probes for high-throughput dynamic fluorescence tracking microscopy on surfaces. ...