Organophosphorus insecticides elicit toxicity by inhibiting acetylcholinesterase. Young animals are generally more sensitive than adults to these toxicants. A number of studies reported that some organophosphorus agents also bind directly to muscarinic receptors, in particular the m(2) subtype, in tissues from adult rats. As both the density and agonist affinity states of cardiac muscarinic rec...

We report a biosensor based on organophosphorus hydrolase (OPH) multilayer modified microcantilever (MCL) for detection of organophosphorus compounds (OPs). The assay is based on substrate-dependent bending of the OPH functionalized MCLs. The cantilever bending amplitude at equilibrium was a function of the concentration of paraoxon with the dynamic range extending from 10(-7) to 10(-3)M. The l...

Organophosphorus hydrolase can effectively degrade organic phosphorus compounds such as sarin. In this study, we constructed a recombinant Bacillus subtilis mutant expressing organophosphorus hydrolase, measured the effect of on degradation rate nerve agent sarin, and selected optimal mutation scheme. Three different genes, 257L, 257Y 303T, were ligated to PMA0911 vector transferred into WB800 ...

Fatty acid amide hydrolase (FAAH) plays an important role in nerve function by regulating the action of endocannabinoids (e.g., anandamide) and hydrolyzing a sleep-inducing factor (oleamide). Several organophosphorus pesticides and related compounds are shown in this study to be more potent in vivo inhibitors of mouse brain FAAH than neuropathy target esterase (NTE), raising the question of the...

Resistance to organophosphorus, carbamate and pyrethroid insecticides in the diamondback moth, Plutella xylostella (L.) is widespread in Japan. Consequently, tertiary amines such as cartap and thiocyclam, microbial insecticides (formulations of Bacillus thuringiensis Berliner) and chitin synthesis inhibitors have been widely used for control. A high level of resistance to these alternative inse...

OBJECTIVE The purpose of this work was to compare DNA damage, acetylcholinesterase (AChE) activity, inflammatory markers and clinical symptoms in farmers exposed to organophosphorus pesticides to individuals that had no pesticide exposure. MATERIALS AND METHODS We conducted a cross-sectional survey with a total of 134 people. The subject group consisted of 67 farmers who were exposed to organ...

Trialkylphosphates are of considerable interest from the military-chemical point view as precursors highly toxic organophosphorus toxicants, possible products their decontamination, complexing agents in processes reprocessing used nuclear fuel, etc. Meanwhile, development ways to identify these substances is still its infancy. In addition, trialkylphosphates an interesting subject for search a ...

Objective The extensive pollution of natural ecosystems has occurred by organophosphorus pesticides, being used for improvement of crop production in agriculture. Biodegradation is interested in application of multifunctional bacteria containing genetical elements which specially degrade organophosphorus compounds. In this paper the detection of two bacteria species, Pseudomonas and Flavobacte...

diazinon (dzn) is a synthetic organophosphorus (ops) insecticide widely used in agricultural and household applications. ops, particularly dzn, are highly lipid soluble liquids. intravenous lipid emulsion (ile) has been shown to reduce toxicity caused by some lipid soluble agents. we evaluated the antidote effect of ile on acute toxicity of dzn. twenty-four sprague-dawley female rats weighting ...