ABSTRACT The genome of Grapevine leafroll-associated virus-5 (GLRaV-5) was cloned, and the sequence of 4766 nt was determined. Degenerate oligonucleotide primers designed from the conserved closterovirus heat shock 70 protein (HSP 70) homologue were used to obtain viral-specific sequences to anchor the cloning of the viral RNA with a genomic walking approach. The partial nucleotide (nt) sequenc...

The complete nucleotide sequence of the maize chlorotic mottle virus (MCMV) genome has been determined to be 4437 nucleotides. The viral genome has four long open reading frames (ORFs) which could encode polypeptides of 31.6, 50, 8.9 and 25.1 kd. If the termination codons, for the polypeptides encoded by the 50 and 8.9 kd ORFs are suppressed, readthrough products of 111 and 32.7 kd result. The ...

Open reading frames (ORFs) 21, 29, 62, 63, and 66 of varicella-zoster virus (VZV) are transcribed during latency in human ganglia. ORF 63 is the most frequently expressed gene, and ORF 62 encodes a transcriptional activator. The mechanisms regulating the expression of these genes are not well understood, although analyses of other alphaherpesviruses indicate a role for chromatin in virus gene r...

Gamma-herpesviruses, Epstein-Barr virus, and Kaposi's sarcoma-associated herpesvirus are important human pathogens, because they are involved in tumor development. Murine gamma-herpesvirus-68 (MHV-68 or gammaHV-68) has emerged as a small animal model system for the study of gamma-herpesvirus pathogenesis and host-virus interactions. To identify the genes required for viral replication in vitro ...

On the basis of observational and experimental evidence, several authors have proposed that contagious yawn is linked to our capacity for empathy, thus presenting a powerful tool to explore the root of empathy in animal evolution. The evidence for the occurrence of contagious yawning and its link to empathy, however, is meagre outside primates and only recently domestic dogs have demonstrated t...

pathogens intrusion. Blackleg (1970, 1995) and the contagious ecthyma (1999) were probably introduced into the country by live ruminants imported from Madagascar (9). Since 2002, importation of live animals from Tanzania has been common, increasing the risk of introducing continental pathogens or vectors as illustrated with outbreaks of East Coast fever in 2003 and 2004 in Grande Comore (10). R...

to have discovered the parasitic micro-organisms of molluscum contagiosum, syphilis, cancer, rabies, smallpox, carcinoma (or at least "cancer"), and a few other diseased conditions. If imagination has run riot among the Entamocbac, and made havoc of the affinities of the Haemosporidia, when it comes to the " Clilainydozoa " it seems to become entirely divorced from reason. The history of protoz...

Asexual taxa are short-lived, suggesting that transitions to asexuality represent evolutionary dead-ends. However, with high rates of clonal origin and coexistence of asexuals and sexuals via selective asymmetries, asexuality may persist in the long term as a result of a dynamic equilibrium between clonal origin and extinction. Few such systems have been studied in detail. Here, we investigate ...

When is a person with a cold contagious? A couple of friends, who are put off when I keep my distance from their sneezing and coughing, insist one is only contagious a week or so before symptoms appear. In the back of my memory is the idea that one remains contagious until a few days *after* primary symptoms disappear. I'm not usually so concerned about this, but I'm coping with a couple of oth...

We argue that the linear static potential is built in into the common procedure of extracting it from lattice Wilson loop measurements. To illustrate the point, we extract the potential by the standard lattice method in a model vacuum made of instantons. A beautiful infinitely rising linear potential is obtained in the case where the true potential is actually flattening. We argue that the flux...