the green fluorescent protein (gfp) was originally isolated from the jellyfish aequorea victoria that fluoresces green when exposed to blue light. gfp protein is composed of 238 amino acids with the molecular mass of 26.9 kd. the gfp gene is frequently used in cellular and molecular biology as a reporter gene. to date, many bacterial, yeast , fungal, plants, fly and mammalian cells, including h...

objective(s): progestrone is a prequisite for pre-implantation angiogenesis and induce decidual angiogenesis. it is unknown the effect of progestrone administration on the endometrium of hyperstimulated mice at pre-implantation time. material and methods: adult female nmri mice were divided in three groups [control group, ovarian stimulated group and progestrone treated mice after ovarian stimu...

the influenza a virus is of global concern for the poultry industry, especially the h5 subtype as it has the potential to become highly pathogenic for poultry and mankind. recently, plant expression systems have gained interest as an alternative for the production of vaccine antigens. the goal of the present study was to investigate the possibility of expressing the ha1 protein in nicotiana tab...

background: acute promyelocytic leukemia (apl) is a unique subtype of acute leukemia. apl is a curable disease; however, drug resistance, early mortality, disease relapse and treatment-related complications remain challenges in apl patient management. one issue underlying these challenges is that the molecular mechanisms of the disease are not sufficiently understood. materials and methods: in ...

conjugated linoleic acids (cla) are natural constituents of meat and dairy products from ruminants, originating from bacterial biohydrogenation in the rumen. cla supplementation increases the health benefits of animal-derived foods. there are inconsistent reports of the effects of dietary cla on chicken’s performance; however, the majority of previous reports cite anti-lipogenic effects of cla....

plasma membrane h+-atpase is a major integral membrane protein with a role in various physiological processes including abiotic stress response. to study the effect of nacl on the expression pattern of a gene encoding the plasma membrane h+-atpase, an experiment was carried out in a completely random design with three replications. a pair of specific primers was designed based on the sequence o...

conclusions traf6 plays an important role in the onset of colon cancer; therefore, drugs targeting it may have great clinical significance in the treatment of this disease. results the positive expression rate of traf6 in tumor tissues was 66.7%, which was significantly higher than in normal paracancerous tissues (18.5%). the positive expression rate of traf6 was significantly correlated with d...

background resistance exercise is recommended as a useful therapeutic tool for the treatment of type 2 diabetes (t2d); however, the frequency of studies is inadequate to establish the precise mechanisms of any association between them. objectives in this study, we aimed to assess the effect of three months of resistance training on tcf7l2 expression in pancreatic tissues, serum insulin and gluc...

background cucumber mosaic virus (cmv) has isometric particles with a diameter of about 28–29 nm. a useful method to recognize regions from mutagenesis in the coat protein (cp) gene are important for particle assembly and the expression of viral in cp expression systems like bacteria such as e.coli or yeast as well as their assembly into virus-like particles (vlp). objectives in this paper, we ...