Dutch elm disease (DED), caused by three fungal species in the genus Ophiostoma, is the most devastating disease of both native European and North American elm trees. Although many tolerant cultivars have been identified and released, the tolerance mechanisms are not well understood and true resistance has not yet been achieved. Here we show that the expression of disease-responsive genes in re...

We evaluated whether Dendroctonus frontalis Zimmermann populations were inßuenced by nontrophic interactions involving commensal mites, their mutualistic bluestain fungus Ophiostoma minus (Hedgc.) H. and P. Sydow, and beetle-mutualistic mycangial fungi. We tested for effects of delayed, nonlinear, or positive feedback fromO. minus and mites onD. frontalis population growth. We predicted that (1...

As part of the DNA Sequencing Research Group of the Association of Biomolecular Resource Facilities, we have tested the reproducibility of the Roche/454 GS-FLX Titanium System at five core facilities. Experience with the Roche/454 system ranged from <10 to >340 sequencing runs performed. All participating sites were supplied with an aliquot of a common DNA preparation and were requested to cond...