نتایج جستجو برای: one step pcr method
تعداد نتایج: 3566334 فیلتر نتایج به سال:
The Agence Nationale de Recherche sur le Sida et les hépatites virales (ANRS) previously developed a widely used method for HIV-1 RNA quantification (Biocentric). Here, we report the development of a new specific and sensitive method for HIV-2 RNA quantification, based on an adaptation of the existing HIV-1 protocol. The new test is based on TaqMan one-step reverse transcription-quantitative PC...
Background: Hepatitis C virus (HCV) has been reported to be the most common cause of chronic viral Hepatitis with increased prevalence rate worldwide. HCV genotype is an important predictive factor in the outcome of HCV treatment, therefore an essential component of clinical practice. Molecular methods are the most common diagnostic tools for HCV genotyping. HCV genotype might also have relevan...
Background: The invention of Polymerase Chain Reaction (PCR) marked a breakthrough in biomedical research. Its divided the timeline into an era before and after PCR. Because its multiple applications, it has become vital tool for clinical researchers diagnosticians. A effective PCR experiment requires adequate knowledge each reaction component stepby- step procedure to attain optimized results....
A novel method eliminating DNA-mediated PCR product formation in reverse transcription PCR (RT-PCR) amplification of specific RNA sequences is described. The method exploits the higher melting temperature values of peptide nucleic acid (PNA)/DNA duplexes compared with DNA/DNA duplexes by binding a sequence-specific PNA probe to a genomic sequence immediately overlapping one of the PCR-primer at...
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