Molecular epidemiology and cross-sectional study. Multicenter from referral centers. 326 women were included. The primary outcome was designed of a kit for screening HPV-genotypes based on the prevalence found in population. Oligonucleotides different genotypes designed. conditions quantitative PCR pools low- high-risk (LR HR) HPVgenotypes established.

While most molecular reactions involving synthetic oligonucleotides, notably PCR and DNA sequencing, are carried out in liquid phase, a rapidly growing number of important applications utilize synthetic oligonucleotides attached to solid supports. In general, the purpose for using a solid support-attached oligonucleotide is to capture something, hold on to it, enrich it, and identify and/or pur...

The coupling of 2-cyanoethyl thymidine phosphoramidite to solid-support-bound, phosphate-unprotected oligothymidylates and their phosphorothioate analogues was studied. The yield of the coupling reaction depended on the pK(BH)()+ values of protonated nitrogen bases that served as counterions to the phosphodiester functions of oligonucleotides. To maximize the coupling efficiency, the oligonucle...

The interaction of phospholipid membranes and oligonucleotides complexed with a positively charged surfactant is reported. Phospholipid membranes were assembled at the interface between an immobilized organic phase and an aqueous phase using the Langmuir-Blodgett (L-B) technique. The interaction and adsorption of the naked oligonucleotides and oligonucleotides complexed with cetylpyridinium chl...

The article is devoted to hidden symmetries in long sequences of oligonucleotides of single stranded DNA. The notions of tetra-groups of oligonucleotides and also collective frequencies and collective probabilities of members of the tetra-groups are introduced to study hidden tetra-group regularities in mentioned sequences. Each of such tetra-group contains 4 members, each of which combines 4^(...

As research in synthetic biology and genomic sciences becomes more widespread, the need for diverse oligonucleotide populations has increased. To limit reagent cost, it would be advantageous to obtain high quality populations in minute amounts. Towards that end, synthesis of DNA strands in capillaries utilizing photolabile 3-nitrophenylpropyloxycarbonyl (NPPOC) chemistry and ultraviolet-light e...

Genetic and biochemical studies have provided convincing evidence that the 5' noncoding region (5' NCR) of hepatitis C virus (HCV) is highly conserved among viral isolates worldwide and that translation of HCV is directed by an internal ribosome entry site (IRES) located within the 5' NCR. We have investigated inhibition of HCV gene expression using antisense oligonucleotides complementary to t...

Short oligonucleotides binding complementary mRNA essential for bacterial growth could be used as antibacterials provided that they are delivered to cells. One promising oligonucleotide is peptide nucleic acid (PNA), a synthetic DNA analog with neutral peptide-like backbone. Unfortunately, bacteria do not import short oligonucleotides, including PNA, from the environment and efficient non-invas...

Abstract Genome-editing tools from Oligonucleotide-Directed Mutagenesis (ODM) to CRISPR system use synthetic oligonucleotides for targeted exchange of nucleotides. Presently, majority genome-editing protocols are dependent on the in vitro cell or tissue culture systems with somaclonal variation, and limitations plant regeneration. Therefore, here, we report an alternative planta cellular test o...

We report here on the analysis of synthetic oligonucleotides by electrospray ionization mass spectrometry (ESI-MS). After intensive removal of salt ions (especially sodium cations), negative ion mass spectra, allowing mass measurement with an accuracy of 0.01%, were obtained on several oligonucleotides up to 80 nucleotides. In most cases, the resolution was sufficient to observe n-1 and n-2 for...