A simple and efficient protocol for the preparative-scale synthesis of various lengths of oligo-N-acetyllactosamine (oligo-LacNAc) and its multi-sialylated extensions is described. The strategy utilizes one thermophilic bacterial thymidylyltransferase (RmlA) coupled with corresponding sugar-1-phosphate kinases to generate two uridine diphosphate sugars, UDP-galactose and UDP-N-acetylglucosamine...

The geology of the Darien province of eastern Panama is presented through a new geologic map and detailed biostratigraphic and paleobathymetric analysis of its Upper Cretaceous to upper Miocene sediments. The sequence of events inferred from the stratigraphie record includes the collision of the Panama arc (the southwestern margin of the Caribbean plate) and South American continent. Three tect...

DNA cleavage by eukaryotic type II DNA topoisomerase (EC 5.99.1.3) was strongly inhibited by an oligonucleotide containing 10 dGua residues. Catalytic activities of topoisomerase II, as measured by relaxation and decatenation reactions, were also inhibited by oligo(dG)10. Inhibition was specific to oligo(dG)10; other oligonucleotides, nucleotides, or single-stranded DNAs tested did not influenc...

The Saccharomyces cerevisiae amino acid transceptor Gap1 functions as receptor for signalling to the PKA pathway and concomitantly undergoes substrate-induced oligo-ubiquitination and endocytosis. We have identified specific amino acids and analogues that uncouple to certain extent signalling, transport, oligo-ubiquitination and endocytosis. L-lysine, L-histidine and L-tryptophan are transporte...

Since the acceptance of plate tectonics, presence calc-alkaline magmatic rocks has been recognized as evidence subduction. But under specific geodynamic circumstances, subduction may occur without generating magmas. Here, we investigate Cenozoic northern Lesser Antilles arc where, from sparsely exposed records, Eocene?Oligocene and Pliocene flare-ups a Miocene lull were postulated. Nevertheless...

The expression of Beta, the single-stranded annealing protein (SSAP) of bacteriophage lambda in Escherichia coli promotes high levels of oligonucleotide (oligo)-mediated mutagenesis and offers a quick way to create single or multiple base pair insertions, deletions, or substitutions in the bacterial chromosome. High rates of mutagenesis can be obtained by the use of mismatch repair (MMR)-resist...

An enzymatic activity which, upon addition of double-stranded RNA (dsRNA), polymerizes ATP into pppA(2’pYA), or 2’5’-oligo(A), is increased in interferon-treated cells. An assay for this enzymatic activity, designated 2’5’-oligo(A) polymerase, is described here. The assay uses DEAE-cellulose chromatography for a direct measurement of 2’5’-oligo(A) synthesized by cell extracts. The optimal condi...

Material Basing on data of 99 patients 14-17 old with JIA boys/ girls 27%/73%, 1group of children attending school 62 (63%), 2 – 37 patients taught at home; among whom 20 (group 2A) on medical indications; 17 (group 2B) at will of the patient and parents. 1 group (syst. variant-14%, poly-50%, oligo-20% , SA-16%), 2 group (syst-27%, poly-49%, oligo-11%., SA-13%), 2-A (syst40%, poly-50%, oligo-5%...