نتایج جستجو برای: of which 17 primers produced polymorphic bands these 17 selected rapd primers produced 233 bands

تعداد نتایج: 21252121  

2011
M. S. I. Sagar M. B. Meah M. M. Rahman A. K. Ghose

Some Trichoderma isolates were collected from different locations of Bangladesh for evaluating their bioefficiency by determining their genetic variations. PCR-based Random Amplified Polymorphic DNA (RAPD) Marker employing 3 decamer primers produced 29 scorable bands of which all (100%) were polymorphic. The co-efficient of gene differentiation (Gst) was 1.0000 reflecting the existence of high ...

Journal: :Nucleic acids research 1992
M F Riedy W J Hamilton C F Aquadro

The random amplified polymorphic DNA (RAPD) method allows the detection of DNA sequence polymorphisms using single primers of arbitrary sequence in the polymerase chain reaction (1). We have attempted to use this technique to assess paternity in a troop of chacma baboons {Papio cynocephalus ursinus). We have also examined individuals from known pedigrees of this species and humans. Our results ...

Journal: :علوم گیاهان زراعی ایران 0
محمد محسن زاده گلفزایی دانشجوی سابق کارشناسی ارشد گیلان حبیب اله سمیع زاده لاهیجی دانشیار دانشکده علوم کشاورزی دانشگاه گیلان علی اعلمی استادیار دانشگاه گیلان مرداویج شعاعی دیلمی رئیس بخش ژنتیک و اصلاح نبات مرکز تحقیقات توتون گیلان سهیلا طالش ساسانی استادیار دانشگاه گیلان

the first step in breeding programs is detecting the genetic diversity in breeding materials. molecular markers help to reduce costs and time in breeding programs. genetic variation in 49 genotypes of tobacco were studied using 12 issr markers, 3 retrotransposon markers and the combination of issr and retrotransposons. one hundred forty seven polymorphic bands were produced using 16 primers. to...

Genetically, every individual is unique; this may stem from inheritance, geographical locations, and/or environmental interactions. This study examined the possibility of developing a cheap and easy-to-use marker that can distinguish among the three ethnic groups in Nigeria using RAPD-PCR. Five RAPD primers, OPA1-3 and OPC1-2, were randomly selected and used to amplify DNA samples isolated from...

2015
T. P. Sasikala

Random amplified polymorphic DNA markers were used to evaluate the genetic diversity in a representative population of Terminalia pallida from six different geographical regions of chittoor district. 20 OPA primers were used for the RAPD analysis. A total of 33 bands yielded from 7 reproducible primers, with a mean of 4.28 amplified bands per primer. The plant species showed 69.5% polymorphism....

2010
Nuhu Alam Jeong Hwa Kim Mi Ja Shim U Youn Lee Tae Soo Lee

This study evaluated the optimal vegetative growth conditions and molecular phylogenetic relationships of eleven strains of Agrocybe cylindracea collected from different ecological regions of Korea, China and Taiwan. The optimal temperature and pH for mycelial growth were observed at 25℃ and 6. Potato dextrose agar and Hennerberg were the favorable media for vegetative growth, whereas glucose t...

Journal: :علوم باغبانی ایران 0
حسن مومنی دانشجوی سابق ارشد دانشگاه شهرکرد بهروز شیران دانشیار، دانشگاه شهرکرد، دانشکده کشاورزی، گروه زراعت و اصلاح نباتات، تخصص: بیوتکنولوژی محمود خدامباشی استادیار دانشگاه شهرکرد کیانوش چغامیرزایی دانشیار دانشگاه رازی

to evaluate the genetic diversity of native populations of crown imperial (fritillaria imperialis l.) in zagross region, issr molecular along with 32 morphological traits were employed. a number of 160 samples corresponding to eight zagross populations distributed in six provinces were collected and assessed. using six primers, 57 bands were totally produced from which 49 were polymorphic. mean...

2007
NABILA TABBASAM YUSUF ZAFAR

Over the last two decades, DNA fingerprinting have been increasingly used to characterize germplasm of different organisms including crop plants, which was otherwise difficult with morphological and or isozyme markers because of paucity of genetic information at phenotypic level. In the present study, two hybrid samples of sorghum (Sorghum bicolor L.) received from Federal Seed Certification an...

Journal: :Genetics and molecular research : GMR 2013
R Basheer-Salimia M Shtaya M Awad J Abdallah Y Hamdan

Until now, neither phenotypic nor molecular approaches have been used to characterize the landraces of Palestine faba beans (Vicia faba). We used PCR-based RAPD markers to determine the genetic diversity and relatedness among 26 Palestinian faba bean landraces (traditional farmers' varieties) from 8 localities in the West Bank, Palestine. In tests with 37 primers, 14 generated no polymorphic ba...

2016
Lallawmsanga Ajit Kumar Passari Vineet Kumar Mishra Vincent Vineeth Leo Bhim Pratap Singh Geetha Valliammai Meyyappan Vijai Kumar Gupta Sivakumar Uthandi Ramesh Chandra Upadhyay

The diversity of wild mushrooms was investigated from two protected forest areas in India and 231 mushroom specimens were morphologically identified. Among them, 76 isolates were screened for their antimicrobial potential against seven bacterial and fungal pathogens. Out of 76 isolates, 45 isolates which displayed significant antimicrobial activities were identified using ITS rRNA gene amplific...

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