The oxidation of a-ketoglutarate in rat liver mitochondria incubated in State 3 and in the uncoupled state in the absence and presence of oligomycin was inhibited by the simultaneous oxidation of fatty acids. Palmitylcarnitine inhibited a-ketoglutarate oxidation by up to 80% and octanoate by up to 30%. Various possibilities for the mechanism of this inhibition were experimentally evaluated. The...

In the initial studies of Lehninger (1) on the oxidation of octanoate by rat liver mitochondria, it was noted that oc-ketoglutarate possessed a unique property in regard to its role as a co-oxidant. Whereas all other intermediates of the tricarboxylic acid cycle t,est,ed for sparking activity required the simultaneous addition of adenosine monophosphate, a-ketoglutarate functioned efficiently b...

By a study of the product-inhibition kinetics of the octanoyl-CoA synthetase from ox liver mitochondria, evidence was obtained consistent with the hypothesis that the enzyme reacts by a Bi Uni Uni Bi Ping Pong type of mechanism in which the order of addition and evolution of substrates and products is CoA, octanoate, octanoyl-CoA, ATP, PP(i) and AMP. There is also evidence that more than one mo...

I have developed a procedure to monitor, by gas chromatography with electron-capture detection, the concentration of vaiproic acid in serum. The valprolc acid Is derivatized into Its phenacyl ester. The assay Is very sensitive, measuring as little as 100 pg of valproic acid easily and accurately. Solvent extraction and concentration of the drug are not necessary; Instead, one only has to precip...

Volatile components in cape gooseberry fruit at ripe stage were collected using headspace-solid phase microextraction, and analyzed by gas chromatography-mass spectrometry. Three solid phase microextraction fiber coatings (DVB/CAR/PDMS, CAR/PDMS, and PDMS/DVB) were tested for evaluation of volatile compounds. DVB/CAR/PDMS fiber showed a strong extraction capacity for volatile compounds and prod...

Background A dry powder inhaled formulation is used for the anti-influenza drug laninamivir octanoate hydrate (laninamivir). Although two successive inhalations (puffs) are recommended to minimize residual amounts of active ingredients, previous reports suggest that pediatric patients with low peak inspiratory flow are unable to inhale the active ingredient adequately. In the present study, we ...