background & aim: the plants of the greenhouse have been affected by infestation of the pest trialeurodes vaporariorum, called greenhouse whitefly, which markedly reduced production. synthetic insecticides have been widely developed and, their extensive use has brought about disadvantages, like environmental disturbance, pest resistance, lethal effects on non target organisms and toxicity t...

phytoplasmal big bud is one of the important diseases of tomato (lycopersicon esculentum) in the world. the disease has been reported from iranian provinces of bushehr, isfahan, fars, khorasane razavi and ardabil. during a summer surrvey in 2010, symptoms of big bud disease was observed in tomato fields of dorood area in lorestan province. characteristic symptoms of the disease were small thick...

Polymerase chain reaction (PCR) is a basic molecular biology technique with a multiplicity of uses, including deoxyribonucleic acid cloning and sequencing, functional analysis of genes, diagnosis of diseases, genotyping and discovery of genetic variants. Reliable primer design is crucial for successful PCR, and for over a decade, the open-source Primer3 software has been widely used for primer ...

piriformospora indica, a cultivable root colonizing endophytic fungus of sebacinales, promotes plant growth and confers resistance against biotic and abiotic stress. the fungus has also the ability to colonize the roots of many varieties of plant species. the goal of present study was to clarify the colonization ability of p.indica in artichoke roots and the respond of plant to water stress. re...

The Stoffel fragment of Taq DNA polymerase lacks the 5' to 3' exonuclease activity that hydrolyzes potentially blocking DNA strands during primer extension. We there-fore asked whether by using this fragment in the PCR, non-extendable, base-paired oligonucleotides could inhibit amplification in a sequence-dependent manner. Model targets were chosen from the partially conserved ribosomal 16S rDN...

Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is an important worldwide pest of citrus. It vectors three phloem-restricted bacteria in the genus Candidatus Liberibacter that cause huanglongbing (citrus greening disease). Studies were conducted to examine the behavioral responses of male and female D. citri to conspecifics of the same and opposite sex, with and without associated citrus host ...

CT140 RFLP Probe: Four primers were designed from the CT140 RFLP probe: PCT140F1, PCT140F2, PCT140R1 and PCT140R2 (Table 1). All four primer combinations gave the strongest bands of 750 bp with Heinz 1706 DNA (Fig. 2). Primer pairs PCT140F1/PCT140R1 and PCT140F2/PCT140R2 gave two bands. Primer pairs PCT140F1/PCT140R2 and PCT140F2/PCT140R1 gave a single band. The primer pair PCT140F2/PCT140R1 ga...

Drought is a major limiting factor of crop productivity worldwide and its incidence is predicted to increase under climate change. Drought adaptation of cool-season grasses is thus a major challenge to secure the agricultural productivity under current and future climate conditions. Endophytes are non-pathogenic plant-associated bacteria that can play an important role in conferring resistance ...