in this study, we investigated the relationship between the genetic polymorphism of growth differentiation factor 9 (gdf9) genes and the litter size in 384 individuals of five breeds of black goats. four pairs of primers were designed to detect single nucleotide polymorphism of gdf9 gene in goats by pcr-sscp. the least square was used to analyze the relation between different genotypes and the ...

Mango is one of the most commercially important fruit crops in tropical and subtropical regions. To increase the efficiency of breeding strategies, two EST-derived marker systems were developed in the present study using information from the mango fruit transcriptome. Using simple sequence repeats, 218 of 230 primer pairs showed stable amplification for 7 mango genotypes with amplicons ranging ...

Using the Anopheles gambiae Giles genome as a template, we designed, screened and identified 14 novel Exon-Primed Intron-Crossing (EPIC) PCR primer pairs for Anopheles pseudopunctipennis Theobald 1901, a major vector of human Plasmodium sp. in South America. These primers were designed to target the conserved regions flanking consecutive exons of different genes and enabled the amplification of...

Twelve polymorphic dinucelotide microsatellites in the freshwater eel Anguilla anguilla L. were isolated and characterized. Genetic diversity was assessed in eels from Lake Constance, Germany. Allele numbers ranged from five to 26 per locus with observed heterozygosities between 0.125 and 0.875. A portion of locus AangCT77 aligns with a transcribed region of the zebrafish gene crystallin beta B...

We report the construction of the first genetic linkage map in the blue mussel, Mytilus edulis. AFLP markers were used in 86 full-sib progeny from a controlled pair mating, applying a double pseudo-test cross strategy. Thirty-six primer pairs generated 2354 peaks, of which 791 (33.6%) were polymorphic in the mapping family. Among those, 341 segregated through the female parent, 296 through the ...

common kilka clupeonella cultriventris (nordmann, 1840) is a brackish water and small pelagic fish species and is one of the most abundant fishes that live gregariously in the caspian sea. a total of 60 specimens of adult common kilka were sampled from two seasons. fifteen pairs of microsatellites previously developed for a.sapidissima, c. pallasi, c.harengus, and s. pilchardus were tested for ...

BACKGROUND AND OBJECTIVE In addition to the well-known phyla Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Spirochaetes, Fusobacteria, Tenericutes, and Chylamydiae, the oral microbiomes of mammals contain species from the lesser-known phyla or candidate divisions, including Synergistetes, TM7, Chlorobi, Chloroflexi, GN02, SR1, and WPS-2. The objectives of this study were to create ...

Nearly every model of DNA computation proposed to date depends upon sequence-specific hybridization operations. In order to better predict the binding specificity of arbitrary deoxyoligonucleotides, a simulator named bind is implemented. Bind operates on a single template DNA sequence and a number of shorter primer sequences. For each primer sequence, bind calculates a theoretical melting tempe...