The properties of Na-Ca-K exchange current through the plasma membrane of intact rod outer segments (ROS) isolated from bovine retinas were studied with the optical probe neutral red. Small cellular organelles such as bovine ROS do not offer an adequate collecting area to measure Na-Ca-K exchange currents with electrophysiological techniques. This study demonstrates that Na-Ca-K exchange curren...

Site-directed mutagenesis of Thr(66) in porcine liver NADH-cytochrome b(5) reductase demonstrated that this residue modulates the semiquinone form of FAD and the rate-limiting step in the catalytic sequence of electron transfer. The absorption spectrum of the T66V mutant showed a typical neutral blue semiquinone intermediate during turnover in the electron transfer from NADH to ferricyanide but...

The molecular filter membrane (MF) was developed for the recovery of small numbers of bacterial contminants in water and air (Goets, 1953a,b; McKee et al., 1953). A number of pathogenic bacteria, including the tubercle bacillus, were grown on these filters by Orlando and Bolduan (1953). These membranes are worthy of further study in the cultivation of mycobacteria. The possible advantages of th...

Foci of cells infected with human cytomegalovirus were noted to stain more intensely than uninfected cells with neutral red, and this provided the basis for development of a plaque assay and plaque reduction neutralization test for cytomegalovirus. Plaques demonstrable by neutral red staining could be counted at 8 days after infection; thus, results could be obtained earlier than for plaque ass...

Neutral hydrogen traces gravitational potentials. In the nearby universe, 21cm emission-line surveys show that the bulk of the HI resides in well-formed, optically-luminous galaxies. At high redshift, 21cm line absorption against background radio quasars occurs in gas-rich systems identified with the highest HI column densities – the " damped Lyman alpha " quasar absorption-line systems. High s...

the objective of the study was to evaluate thecytotoxic effects of aqueous fruit extract of cucumis melo in human prostate cancer cell line (pc-3) using mttand neutral red assays. the crude aqueous extract of c.melo was prepared by cold maceration method, filtered,concentrated and tested on pc-3 cell line. dose-dependent cytotoxic activitieswere exhibited by human prostate carcinoma pc-3 cell l...