نتایج جستجو برای: neurospora
تعداد نتایج: 5175 فیلتر نتایج به سال:
The clock gene period-4 (prd-4) in Neurospora was identified by a single allele displaying shortened circadian period and altered temperature compensation. Positional cloning followed by functional tests show that PRD-4 is an ortholog of mammalian checkpoint kinase 2 (Chk2). Expression of prd-4 is regulated by the circadian clock and, reciprocally, PRD-4 physically interacts with the clock comp...
We show that firefly luciferase is a stable protein when expressed at 25 °C in Neurospora, which limits its use as transcription reporter. We created a short-lived luciferase by fusing a PEST signal to its C-terminus (LUC-PEST) and applied the LUC-PEST reporter system to record in vivo transcription dynamics associated with the Neurospora circadian clock and its blue-light photosensory system o...
Parts of the primary structure of the NAD-specific glutamate dehydrogenase [L-glutamate:NAD oxidoreductase (deaminating), EC 1.4.1.2] from Neurospora crassa are presented. Segments of the sequence representing 886 unique amino-acid residues have been determined; the largest contains 267 residues. There are only short regions of possible homology between this enzyme and the glutamate dehydrogena...
ADP/ATP carrier protein was synthesized in heterologous cell-free systems programmed with Neurospora poly(A)-containing RNA and homologous cell-free systems from Neurospora. The apparent molecular weight of the product obtained in vitro was the same as that of the authentic mitochondrial protein. The primary translation product obtained in reticulocyte lysates starts with formylmethionine when ...
OLLOWING the extensive analysis of the gene-enzyme relationships of the Ftryptophan pathway in bacteria (YANOFSKY and LENNOX 1959; BLUME and BALBINDER 1966) in Saccharomyces and in Neurospora (BONNER, DE Moss and MILLS 1965), it has become O€ interest to examine the pathway in the homothallic ascomycete Aspergillus nidulans (Eidam) Wint. This paper describes the complementation behaviour of try...
Introduction The complete genome sequence of the filamentous fungus Neurospora crassa has been difficult to assemble, because a large amount of AT-rich “genomic dark matter” is lost from traditional shotgun, cosmid, and BAC libraries (1-3). This situation is typical of most repeat-rich eukaryotic genomes. Various novel direct sequencing approaches promise to reduce some of these errors. However...
QDE-1 is an RNA- and DNA-dependent RNA polymerase that has functions in the RNA silencing and DNA repair pathways of the filamentous fungus Neurospora crassa. The crystal structure of the dimeric enzyme has been solved, and the fold of its catalytic core is related closely to that of eukaryotic DNA-dependent RNA polymerases. However, the specific activities of this multifunctional enzyme are st...
Henney, H. (The University of Texas, Austin) and R. Storck. Nucleotide composition of ribonucleic acid from Neurospora crassa. J. Bacteriol. 85:822-826. 1963.-The nucleotide composition of total, ribosomal, and soluble ribonucleic acid (RNA) from the conidia and the hyphae of Neurospora crassa was determined. The corresponding RNA classes from the two morphological types had the same base ratio...
Genomic DNA sequence was determined for the putative Neurospora crassa ABC transporter NCU09975 from several different classical mutant strains including several acriflavin resistant mutants. The sensitivity of these strains to acriflavin was tested. While the open reading frame NCU09975 has multiple polymorphisms in strains sequenced for other purposes, none of the acriflavin resistant classic...
The process designated RIP (repeat-induced point mutation) alters duplicated DNA sequences in the sexual cycle of Neurospora crassa. We tested whether non-Neurospora sequences are susceptible to RIP, explored the basis for the observed immunity to this process of a diverged tandem duplication that probably arose by a natural duplication followed by RIP (the Neurospora zeta-eta region), and inve...
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