نتایج جستجو برای: nested rt pcr
تعداد نتایج: 222624 فیلتر نتایج به سال:
The aim of this study was to evaluate thyroid peroxidase (TPO) mRNA expression in peripheral blood of patients with benign and malignant thyroid disease. Included were 120 thyroid cancer patients, 85 patients with goitre or Graves' disease (GD) and 54 healthy volunteers. TPO mRNA expression was analysed in peripheral blood by nested RT-PCR. In cancer patients, RT-PCR results were compared with ...
The recent outbreaks of West Nile virus (WNV) in the northeastern United States and other regions of the world have made it essential to develop an efficient protocol for surveillance of WNV. In the present report, we describe a high-throughput procedure that combines automated RNA extraction, amplification, and detection of WNV RNA. The procedure analyzed 96 samples in approximately 4.5 h. A r...
BACKGROUND The aim of this study was to analyze whether the CK20 reverse transcriptase polymerase chain reaction (RT-PCR) is suitable for detecting circulating tumor cells and residual tumor cells in lymph nodes, in patients with muscle invasive transitional cell carcinoma (TCC) of the bladder, and to compare these results with standard histological staging. PATIENTS AND METHODS The nested RT...
Background & Objective: Hepatitis E Virus (HEV) infection may be common in Human Immunodeficiency (HIV-1) patients and lead to chronic as well cirrhosis. We intended determine the incidence of HEV among HIV-1 comparison individuals without infection.Methods: In our cross-sectional study, 87 HIV-1-positive were compared 93 healthy Kerman, Iran. Plasma peripheral blood mononuclear cells (PBMCs) o...
The presence of hemoglobin in samples are considered an important inhibitory factor for polymerase chain reaction (PCR). The aim of this study was to examine the influence of red blood cells (RBC)s in cerebrospinal fluid (CSF) as an inhibitory factor to reverse transcription polymerase chain reaction (RT-PCR) for enteroviruses (EV). Forty-four CSF samples from patients showing characteristics o...
OBJECTIVE To identify transcriptionally active open reading frames (ORFs), predicted by bioinformatics, within RD1 genomic segment of Mycobacterium tuberculosis using reverse transcription-polymerase chain reaction (RT-PCR). MATERIALS AND METHODS M. tuberculosis H37Rv was grown in Middlebrook 7H9 medium for 8 weeks and total RNA was isolated using standard procedures. The cDNA was synthesized...
BACKGROUND AND OBJECTIVE Ewing's tumors (ET) are primary malignancies of bone and soft tissues characterized in at least 96% of cases by specific fusion transcripts originating from recurrent chromosomal translocations. Clinical protocols for high-risk metastatic ETs include high-dose radiation/chemotherapy followed by autologous peripheral blood stem cell (PBSC) reinfusion. We used nested reve...
چکید ه سابقه و هدف ویروس نقص ایمنی اکتسابی تیپ یک( HIV-1 ) عامل ایجاد کننده سندرم نقص ایمنی اکتسابی انسانی”ایدز” می‌باشد. انتقال بیماری از طریق انتقال خون در دوره پنجره، در مراکز انتقال خون یک معضل جهانی محسوب می‌شود. علاوه بر این، نیاز مبرمی برای تشخیص سریع، حساس و دقیق عفونت HIV-1 قبل از ظهور آنتی‌بادی در بدن فرد آلوده در بیمارستان‌ها و مراکز بهداشتی احساس می‌شود. تشخیص ژنوم ویروس HIV-1...
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