نتایج جستجو برای: nested pcrs

تعداد نتایج: 31256  

1992
Kazuhiko Fukuda Makoto Watanabe Masao Nitoh Yuh Maruko Youji Suzuki

Eggplants sortirtg plant has been constructed by using image processors, programable contra1 lers and I'A pcrs~lal computers. In order t m a s u r e and analyze an eggplant" size and shape quality, high

Journal: :Irish medical journal 2009
B McGowan K Bennett M Barry M Owens M O'Connor

There are approximately 6,300 people in Ireland with a diagnosis of Chronic Obstructive Pulmonary Disease (COPD) and with a fast growing elderly population the incidence of COPD is likely to increase. This study examines the prescribing patterns of medicines dispensed for the management Asthma/COPD in patients over the age of 35 years using the HSE-Primary Care Reimbursement Services (PCRS) pre...

2017
Jean‐Pierre Jacus

INTRODUCTION Results from studies on awareness disorders in Alzheimer's disease (AD) are controversial because the methodologies, the "objects" of awareness, and the patients' pathologic stage all vary. Our study aimed to compare scores and correlates of awareness according to the stage of the disease and the assessment method. METHODS We compared 20 mild AD patients to 20 mild cognitive impa...

2015
L. Mukundan O.V. Lie L.D. Leary A.M. Papanastassiou L.C. Morgan C.Á. Szabó

We evaluated the spatiotemporal distribution of photic driving (PDR), photoparoxysmal (PPR), and photoconvulsive (PCR) responses recorded by intracranial electrodes (ic-EEG) in a patient with generalized photosensitivity and right frontal lobe cortical dysplasia. Intermittent light stimulation (ILS) was performed thirteen times in nine days. Cortical responses to ILS recorded by ic-EEG were rev...

Journal: :BioTechniques 2004
Chengming Wang Dongya Gao Alexander Vaglenov Bernhard Kaltenboeck

We developed a one-step real-time duplex reverse transcription PCR (RT-PCR) method using the LightCycler platform. This method allows simultaneous reverse transcription and real-time PCR amplification of two mRNAs of specific genes of interest (analyte genes) and mRNA of constantly transcribed genes (housekeeping genes) in a single-tube reaction. Specimen total nucleic acids were used because e...

2016
Daifallah M. Alrazeeni Sikender A. Sheikh Abdulmajeed Mobrad Mazen Al Ghamdi Nabeel Abdulqader Mohammed Al Gabgab Majed Al Qahtani Bader Al Khaldi

OBJECTIVES To determine the epidemiology of non- transported emergency medical services (EMS) calls within the EMS system at Riyadh, Saudi Arabia, to identify factors that contributes to non-transport of patients by EMS provider, and to recommend suggestions for reduction in number of non-transported calls.  METHODS Retrospective analysis of 1390 patient care reports (PCR) of non-transported ...

Journal: :Croatian medical journal 2010
Wei Ling Yu Klaus Nielsen

Here we present a review of most of the currently used polymerase chain reaction (PCR)-based methods for identification of Brucella bacteria in biological samples. We focused in particular on methods using single-pair primers, multiplex primers, real-time PCRs, PCRs for marine Brucella, and PCRs for molecular biotyping. These methods are becoming very important tools for the identification of B...

Journal: :Gene 1990
M C Longo M S Berninger J L Hartley

Polymerase chain reactions (PCRs) synthesize abundant amplification products. Contamination of new PCRs with trace amounts of these products, called carry-over contamination, yields false positive results. Carry-over contamination from some previous PCR can be a significant problem, due both to the abundance of PCR products, and to the ideal structure of the contaminant material for re-amplific...

Journal: :Journal of clinical microbiology 2011
Justine Cheval Virginie Sauvage Lionel Frangeul Laurent Dacheux Ghislaine Guigon Nicolas Dumey Kevin Pariente Claudine Rousseaux Fabien Dorange Nicolas Berthet Sylvain Brisse Ivan Moszer Hervé Bourhy Claude Jean Manuguerra Marc Lecuit Ana Burguiere Valérie Caro Marc Eloit

High-throughput sequencing furnishes a large number of short sequence reads from uncloned DNA and has rapidly become a major tool for identifying viruses in biological samples, and in particular when the target sequence is undefined. In this study, we assessed the analytical sensitivity of a pipeline for detection of viruses in biological samples based on either the Roche-454 genome sequencer o...

2012
Srey V. Horm Ramona A. Gutiérrez San Sorn Philippe Buchy

BACKGROUND Very little is known regarding the persistence of highly pathogenic avian influenza H5N1 viruses in natural settings during outbreaks in tropical countries, although environmental factors may well play a role in the persistence and in the transmission of H5N1 virus. OBJECTIVE To investigate various environmental compartments surrounding outbreak areas as potential sources for H5N1 ...

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