Human buccal cells were found to vary in their day-to-day capacity to support attachment of certain strains of gonococci. Gonococci serially cultured in vitro also differed in their day-to-day ability to attach to buccal cells.

Penicillinase-producing Neisseria gonorrhoeae (PPNG) carrying the blaTEM-135 gene is of particular concern, as it is considered a stepping stone toward resistance to extended-spectrum cephalosporins. Here, we sought to characterize plasmid types and the occurrence of the blaTEM-135 gene for N. gonorrhoeae clinical isolates from Australia. We found that blaTEM-135 was prevalent in Australian PPN...

Financial support was provided by the National Science Foundation (NSF) East Asian Pacific Summer Institute program IIA-1415092, NSF grant DEB-1115895 and DEB-1336290, National Speleological Society Rapid Response Fund, US Fish and Wildlife Service, National Science and Technology Foundation grant no. 2013FY113600, The Robert and Patricia Switzer Foundation, and the crowd-funding platform of Ex...

A multitarget PCR was developed for the direct detection of penicillinase-producing Neisseria gonorrhoeae (PPNG). The assay was validated by testing 342 PPNG isolates and 415 clinical samples. The method is suitable for routine detection of PPNG strains. Its multitarget approach reduces the potential for false-negative results caused by sequence variations.

Antimicrobial-resistant Neisseria gonorrhoeae is a major public health concern. The surveillance of antimicrobial resistance benefits from rapid and accurate molecular techniques in diagnostics to facilitate individualised medicine stewardship. To support the recommendations for empirical treatment gonococcal infections Bulgaria, we investigated N. gonorrhoeae-positive clinical specimens 2018 2...

A lectin slide agglutination test has been developed for the confirmatory identification of Neisseria gonorrhoeae. With wheat germ lectin as an agglutinin, 164 of 165 clinical isolates of N. gonorrhoeae gave a 3 to 4+ reaction within 6 to 8 min. Four gonococcal isolates, even though negative by the fluoresecent-antibody method, gave strong positive reactions with the wheat germ lectin. Among 23...