Some isolates of bacteria were taken from various places inside the hospital, including operating theaters,corridors, ventilation areas, floors, and other samples patients suffering antibioticresistance.The study aims to discover multiple resistant present in hospital environment, theextent their resistance, detect gene NDM-1, NDM-2, which when they are present, bacteriaare highly resistant.Whe...

OBJECTIVES Characterize a NDM-1 producing K. pneumoniae isolate recovered from a patient hospitalized in Guadeloupe, French West Indies, after its transfer from Cuba METHODS Antibiotic susceptibilities were determined by the disk diffusion method, and E-test. Carbapenemase production was assessed using the Carba NP test. Antibiotic resistance determinants and their surrounding structures were...

Neonatal diabetes mellitus (NDM) is the term commonly used to describe diabetes with onset before 6 months-of-age. It occurs in approximately one out of every 100,000-300,000 live births. Although this term encompasses diabetes of any etiology, it is recognized that NDM diagnosed before 6 months-of-age is most often monogenic in nature. Clinically, NDM subgroups include transient (TNDM) and per...

The New Delhi Metallo-β-lactamase (NDM-1) gene makes multiple pathogenic microorganisms resistant to all known β-lactam antibiotics. The rapid emergence of NDM-1 has been linked to mobile plasmids that move between different strains resulting in world-wide dissemination. Biochemical studies revealed that NDM-1 is capable of efficiently hydrolyzing a wide range of β-lactams, including many carba...

New Delhi Metallo-β-lactamase 1 (NDM-1) is a monomeric, plasmid-encoded enzyme that can hydrolyze β-lactam antibiotics over a wide range. Presence of this enzyme renders the host bacteria immune to carbapenems, the so-called last resort drugs, as reported in Klebsiella pneumonia, Escherichia coli and Acenetobacter baumanii. Multi-drug resistance of nosocomial pathogens as above possess a seriou...

Drug resistance has mushroomed up after advent of every major class of antimicrobial drugs, varying in time from as short as 1 year (penicillin) to >10 years. Organisms associated with nosocomial and community acquired infections are also becoming drug resistant due to the over utilization of antibiotics, consequently leading to high morbidity, mortality and increased health costs. One such exa...

Multidrug resistant (MDR) Klebsiella pneumoniae is a common cause of nosocomial infections worldwide. Recent years have seen an explosion of resistance to extended-spectrum β-lactamases (ESBLs) and emergence of carbapenem resistance. Here, we examine 198 invasive K. pneumoniae isolates collected from over a decade in Kilifi County Hospital (KCH) in Kenya. We observe a significant increase in MD...

a rapid and sensitive hplc method has been developed for the quantification of mirtazapine (mrz), a noradrenergic and specific serotonergic inhibitor antidepressant (nassa) and its two major metabolites n-desmethylmirtazapine (ndm) and 8-hydroxymirtazapine (8-ohm) in human plasma. the separation was achieved using chromolith c18 column and a mobile phase of acetonitrile: phosphate buffer (ph=3,...

Objective(s): Infections due to carbapenemase-producing Klebsiella pneumoniae are associated with high morbidity and mortality. In this study, we report a hospital outbreak due to co-producing OXA-48 and NDM-1 K. pneumoniae clone. The aim of the study is to investigate the clonal relationship of strains, risk factors of outbreak and infection control measures.<em...

Abstract Background Carbapenem-resistant Acinetobacter baumannii is considered a top priority pathogen by the World Health Organization for combatting increasing antibiotic resistance and development of new drugs. Since it was originally reported in Klebsiella pneumoniae 2009, quick spread bla NDM-1 gene encoding New-Delhi metallo-beta-lactamase-1 (NDM-1) increasingly recognized as serious thre...