This is a Health Evidence Network (HEN) synthesis report on the most effective and cost-effective interventions in alcohol control. Evidence shows the most effective approach is to implement a combination of: increases in alcohol prices, reduction in the availability of alcohol, and measures against drunk driving and underage drinking. A report from the Health Evidence Network reviews the evide...

Inteins are parasitic genetic elements that excise themselves at the protein level by self-splicing, allowing the formation of functional, nondisrupted proteins. Many inteins contain a homing endonuclease (HEN) domain and rely on its activity for horizontal propagation. However, successful invasion of an entire population will make this activity redundant, and the HEN domain is expected to dege...

The recombinant antibodies AG275, AG294 and AG274 detect the hen egg-white lysozyme by ELISA.

The recombinant ovalbumin (OVA) produced in yeast Pichia pastoris was purified from the culture medium by anion exchange chromatography, and its structural characteristics were compared with those of hen egg OVA, mainly from the point of view of posttranslational modification. The expressed OVA consisted of two molecular species immmunoreactive with antibody for hen egg OVA. The two molecular s...

The target size of neurotoxic esterase (NTE), the putative target site for the initiation of organophosphorus-compound-induced delayed neurotoxicity, and acetylcholinesterase (AChE) from hen brain were examined by determining the rate at which the activities of the esterases were destroyed by ionizing irradiation. Samples of hen brain were prepared by slowly drying a microsomal preparation unde...

This is a Health Evidence Network (HEN) synthesis report on injuries in children and older people. The evidence of proven and promising strategies for the prevention of unintentional injuries in children and the older people is considerable. Legislative, environmental modification and educational approaches all have a part to play in preventing or reducing childhood injuries, and their interact...

Heat inactivation was studied at 45, 50, 55, and 60 degrees for all of the phenyl valerate hydrolases (PVase), including neurotoxic esterase (NTE) and inhibitor-resistant esterase (IRE), in homogenates of hen or rat brain or in preparations of hen brain microsomal membranes. Hen and rat brain homogenates were prepared in buffer (50 mM Tris/0.20 mM EDTA, pH 8.00, at 25 degrees). Hen brain micros...

Optimization of carnosine and anserine extraction from chicken breast was performed using response surface methodology (RSM) to obtain the maximized physiological activities for anti-glycation and anti-oxidation. The optimum extraction conditions were water extraction for 1.6 h in the case of the 20-wk laying hen muscle and water extraction for 2.12 h in the case of 90-wk laying hen muscle. Hig...

Samenvatting Economisch daklozen zijn mensen die vaak wel een baan maar geen huis hebben. Ze zoeken met spoed woonruimte. Op de huidige krappe woningmarkt er voor hen mogelijkheden en in opvang is plaats. In vier praktijkgevallen – drie steden regio wordt gezocht naar oplossingen deze groep mensen. Dat lukt, op kleine schaal. Voor hen, ook andere spoedzoekers, biedt ander, aanvullend type wonin...

Toll-like receptors (TLR) activate multiple steps in inflammatory reactions in innate immune responses. They also activate signals that are critically involved in the initiation of adaptive immune responses. Many tumorigenic chemicals have been associated with endotoxin hypersensitivity mediated through TLR4. To determine the role of TLR4 in cutaneous skin carcinogenesis, we treated TLR4-defici...