An efficient protocol providing a dual regeneration pathway via direct shoot organogenesis and somatic embryogenesis for an endangered species, Metabriggsia ovalifolia W. T. Wang, was established from leaf explants. When applied at 2.5 μM, the cytokinins 6-benzyladenine (BA) or thidiazuron (TDZ) and the auxins indole-3-butyric acid (IBA), α-naphthaleneacetic acid (NAA) and indole-3-acetic acid ...

Dendrobium phalaenopsis Fitzg. (also known as the Larat orchid) is an endemic orchid from Island, Eastern Indonesia. Its beautiful flowers mean that many plants are taken for commercial purposes, leading to rapid decline of populations in their natural habitats. The objectives this study were determine which organs transgenic carrying 35S::GR::AtRKD4 construct, together with concentrations plan...

Rose is the most favorite cut flowers all over the world. Production of high-quality flowers, prevention, and delay of flower senescence, is a major goal in floriculture. Now a day, biotechnological approaches have been used to improve ornamental attributes. Tissue culture and genetic transformation appear to offer valuable advancements for operating floral characteristics. In this study, after...

Cotyledonary segments from the germinated immature zygotic embryos were used for somatic embryogenesis of red sandalwood (Pterocarpus santalinus). The explants established on Murashige and Skoog (MS) medium containing 5% sucrose combination 6-benzylaminopurine (BAP), 2,4-Dichlorophenoxyacetic acid (2,4-D), α-Naphthaleneacetic acid. All treatments responded to callus induction with a 36–97% freq...

In vitro rooting as one of the most critical steps micropropagation is affected by various extrinsic (e.g., medium composition, auxins) and intrinsic factors species, explant). Passiflora in adventitious a difficult, complex, non-linear process. Since multivariable complex biological process, efficient reliable computational approaches such machine learning (ML) are required to model, predict, ...

Transgenic technology is an excellent alternative for improvement of crop production and disease free crops such as potato, which is one of the most important crops worldwide. One of the first steps to apply the transgenic technique is the establishment of an efficient plant regeneration protocol. This is a limiting step in this kind of study, since most protocols are species-specific and some ...

Lopezia racemosa Cav. is a plant used in Mexican traditional medicine to heal inflammatory diseases. From this plant we isolated the novel compound 6-O-palmitoyl- 3-O-β-D-glucopyranosylcampesterol (1) and 6-O-palmitoyl-3-O-β-D-glucopyranosyl-β-sitosterol (2), previously reported to have cytotoxic activity on several cancer cell lines. We evaluated the anti-inflammatory activity of 1 in vivo by ...

Polar auxin transport is essential for the formation of continuous vascular strands in the plant body. To understand its mechanism, polar auxin transport inhibitors have often been used. However, the role of auxin in vascular differentiation at the unicellular level has remained elusive. Using a Zinnia elegans cell culture system, in which single mesophyll cells transdifferentiate into trachear...