Solid-state nanopores have been used to perform measurements at the single-molecule level to examine the local structure and flexibility of nucleic acids, the unfolding of proteins, and binding affinity of different ligands. By coupling these nanopores to the resistive-pulse technique, such measurements can be done under a wide variety of conditions and without the need for labeling. In the res...

Membrane Transfer Process for the Creation of Low-Noise Solid State Nanopore Devices Tarun Jain Nanopore sensors are an emerging technology whereby the modulation of a current trace upon passage of the analyte through the nanopore is used to infer the properties of the analyte. This technology is particularly attractive because it is label-free, rapid, and intrinsically single molecule. However...

Nanopore-based sequencing has demonstrated a significant potential for the development of fast, accurate, and cost-efficient fingerprinting techniques for next generation molecular detection and sequencing. We propose a specific multilayered graphene-based nanopore device architecture for the recognition of single biomolecules. Molecular detection and analysis can be accomplished through the de...

Driving single nanoparticles like DNA and proteins by the electrophoretic force through a nanopore in the presence of an electric field produces an ionic current, which passes from the nanopore. The current blockage due to the nanoparticle translocation makes an observable change in the ionic current, which is useful for the study of bio molecules. Many efforts are made to perceive the electro ...

Carbon molecular sieves were prepared from petroleum coke by chemical activation. The effects of activation temperature, holding time, preliminary treatments and activated agent ratio on surface area, pore structure of the carbon molecular sieves produced in our lab were investigated. The specific surface area and average pore size of the carbon are obviously increased with increasing use of ac...

High-bandwidth and low-noise nanopore sensor and detection electronics are crucial in achieving single-DNA-base resolution. A potential way to accomplish this goal is to integrate solid-state nanopores within a CMOS platform, in close proximity to the biasing electrodes and custom-designed amplifier electronics. Here we report the integration of solid-state nanopore devices in a commercial comp...

The assembly of a DNA-DNA polymerase binary complex is the precursory step in genome replication, in which the enzyme binds to the 3' junction created when a primer binds to its complementary substrate. In this study, we use an active control method for observing the binding interaction between Klenow fragment (exo-) (KF) in the bulk-phase chamber above an α-hemolysin (α-HL) nanopore and a sing...

Co-axial electrospinning is an efficient technique to develop core-shell or hollow nanofibrous structures. In this study electrospun carbon nanofibers with three different morphologies, i.e. solid porous structure (P-ECNF), wall (H-ECNF), and (HP-ECNF) were developed through bicomponent co-axial of polyacrylonitrile (PAN) poly (methyl methacrylate) (PMMA) by varying proportion the sacrificial P...

Two DNA bases, 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (hmC), marks of epigenetic modification, are recognized in immobilized DNA strands and distinguished from G, A, T and C by nanopore current recording. Therefore, if further aspects of nanopore sequencing can be addressed, the approach will provide a means to locate epigenetic modifications in unamplified genomic DNA.

The enzymatic degradation of long polysaccharide chains is monitored by nanopore detection. It follows a Michaelis-Menten mechanism. We measure the corresponding kinetic constants at the single molecule level. The simulation results of the degradation process allowed one to account for the oligosaccharide size distribution detected by a nanopore.