Nanopores have become a subject of interest in the scientific community due to their potential uses in nanometer-scale laboratory and research applications, including infectious disease diagnostics and DNA sequencing. Additionally, they display behavioral similarity to molecular and cellular scale physiological processes. Recent advances in information theory have made it possible to probe the ...

The nanopore sensor as a high-throughput and low-cost technology can detect a single molecule in a solution. In the present study, relatively large silicon nitride (Si3N4) nanopores with diameters of ∼28 and ∼88 nm were fabricated successfully using a focused Ga ion beam. We have used solid-state nanopores with various sizes to detect the single horseradish peroxidase (HRP) molecule and for the...

This paper describes the electrical detection of single-stranded DNA (ssDNA) at the single molecule level using an engineered alamethicin (Alm) channel embedded in bilayer lipid membranes (BLMs). Nanopore sequencing has the potential to become a direct, fast and inexpensive DNA sequencing methodology (Fig. 1). We propose here the chemically covalent dimmers of Alm (di-Alm) in which monomers wer...

Submitted for the MAR10 Meeting of The American Physical Society Controlled Reverse Translocation of DNA Through a Solid-State Nanopore1 PAUL WEINGER, VENKAT BALAGURUSAMY, SUNGCHEOL KIM, XINSHENG SEAN LING, Brown University — Reverse DNA translocation [1] is a process in which a DNA is pulled out of a nanopore against the forward electric force. In this process, the effective diffusion constant...

Nanopore sensing is a versatile technique for the analysis of molecules on the single-molecule level. However, extracting information from data with established algorithms usually requires timeconsuming checks by an experienced researcher due to inherent variability of solid-state nanopores. Here, we develop a convolutional neural network (CNN) for the fully automated extraction of information ...

The diffusion-influenced translocation behavior of individual nanoparticles upon passage through a conical nanopore has been elucidated by using a pressure-reversal, resistive-pulse technique, as reported by Lan and White in this issue of ACS Nano. We outline here some recent progress in conical nanopore analysis, and we present some prospects for future developments. Compared to cylindrical na...

A simple theoretical model based on electric-field-assisted growth and dissolution of the barrier oxide has been proposed to understand the mechanisms for formation of a one-dimensional 1D anodic aluminum oxide AAO nanopore array. It is shown that formation of a one-dimensional horizontal nanopore array is the natural result of the self-adjusting effect of anodic oxidation. Because the electric...

Solid-state nanopores have emerged as a versatile tool for the characterization of single biomolecules such as nucleic acids and proteins. However, the creation of a nanopore in a thin insulating membrane remains challenging. Fabrication methods involving specialized focused electron beam systems can produce well-defined nanopores, but yield of reliable and low-noise nanopores in commercially a...

The authors have addressed theoretically the hydrodynamic effect on the translocation of DNA through nanopores. They consider the cases of nanopore surface charge being opposite to the charge of the translocating polymer. The authors show that, because of the high electric field across the nanopore in DNA translocation experiments, electro-osmotic flow is able to create an absorbing region comp...

The influence of an electric field on an isolated channel or nanopore separating two compartments filled with electrolytes produces a constant ion flux through the pore. Nucleic acids added to one compartment traverse the pore, and modulate the current in a sequence-dependent manner. While translocation is faster than detection, the α-hemolysin nanopore (α-HL) successfully senses base modificat...