background and objectives: a new real- time pcr protocol for the detection of trichomonas vaginalis in pooled urine samples has been optimized and validated. materials and methods: the amplification protocol, targeting a 2kb repeated gene in the t. vaginalis genome, was optimized by varying pcr parameters. as a reference method, a real-time pcr protocol targeting the beta-tubulin gene (y. versl...

The use of animal models in modeling of human genetic disease has many advantages. In some cases, however, this method may not be applicable due to some limitations, such as differences in tissue composition, anatomy and physiology of humans and animals. Isogenic human disease models are a population of cells that are selected or engineered to model a specific genetic disease, in vitro. They ar...

We study two types of targeting in this paper, exogenous targeting and endogenous targeting. By exogenous targeting we mean targeting by product preferences: advertising levels and prices geared to specific consumer preferences. Exogenous targeting presumes observability of consumer preferences (or of factors correlated with them). This is the sort of targeting that is much in the news in conte...

Abstract Purpose: our goal is to reduce the release rate of methotrexate (MTX) and increase cell death efficiency. Methods: Carboxylated multi-walled carbon nanotubes (MWCNT-COOH) were functionalized with MTX as a cytotoxic agent, FA as a targeting moiety and polyethylene amine (PEI) as a hydrophilic agent. Ultimately, MWCNT-MTX and MWCNT-MTX-PEI-FA were synthesized. Methotrexate re...

Abstract Purpose: our goal is to reduce the release rate of methotrexate (MTX) and increase cell death efficiency. Methods: Carboxylated multi-walled carbon nanotubes (MWCNT-COOH) were functionalized with MTX as a cytotoxic agent, FA as a targeting moiety and polyethylene amine (PEI) as a hydrophilic agent. Ultimately, MWCNT-MTX and MWCNT-MTX-PEI-FA were synthesized. Methotrexate re...