نتایج جستجو برای: nad dependent

تعداد نتایج: 703138  

Journal: :The Journal of biological chemistry 2004
Jonathan V Rocheleau W Steven Head David W Piston

Glucose-stimulated insulin secretion is a multistep process dependent on beta-cell metabolic flux. Our previous studies on intact pancreatic islets used two-photon NAD(P)H imaging as a quantitative measure of the combined redox signal from NADH and NADPH (referred to as NAD(P)H). These studies showed that pyruvate, a non-secretagogue, enters beta-cells and causes a transient rise in NAD(P)H. To...

Journal: :Plant physiology 1974
P J Gruber S E Frederick N E Tolbert

Cell-free extracts of Chlorella pyrenoidosa contained two enzymes capable of oxidizing d-lactate; these were glycolate dehydrogenase and NAD(+)-dependent d-lactate dehydrogenase. The two enzymes could be distinguished by differential centrifugation, glycolate dehydrogenase being largely particulate and NAD(+)-d-lactate dehydrogenase being soluble. The reduction of pyruvate by NADH proceeded mor...

Journal: :Plant physiology 1992
C A McIntosh D J Oliver

The NAD(+)-dependent isocitrate dehydrogenase from etiolated pea (Pisum sativum L.) mitochondria was purified more than 200-fold by dye-ligand binding on Matrix Gel Blue A and gel filtration on Superose 6. The enzyme was stabilized during purification by the inclusion of 20% glycerol. In crude matrix extracts, the enzyme activity eluted from Superose 6 with apparent molecular masses of 1400 +/-...

Journal: :The Biochemical journal 1982
R H Jackson J A Cole A Cornish-Bowden

The kinetic characteristics of the diaphorase activities associated with the NADH-dependent nitrite reductase (EC 1.6.6.4) from Escherichia coli have been determined. The values of the apparent maximum velocity are similar for the reduction of Fe(CN)6(3)-and mammalian cytochrome c by NADH. These reactions may therefore have the same rate-limiting step. NAD+ activates NADH-dependent reduction of...

2005
Shintaro Kinugawa Juhua Zhang Eric Messina Erin Walsh Harer Huang Pawel M. Kaminski Michael S. Wolin Thomas H. Hintze

Kinugawa, Shintaro, Juhua Zhang, Eric Messina, Erin Walsh, Harer Huang, Pawel M. Kaminski, Michael S. Wolin, and Thomas H. Hintze. gp91-containing NAD(P)H oxidase mediates attenuation of nitric oxide-dependent control of myocardial oxygen consumption by ANG II. Am J Physiol Heart Circ Physiol 289: H862–H867, 2005. First published March 18, 2005; doi:10.1152/ajpheart.00076.2005.—We have previous...

2000
Barbaros NALBANTOĞLU

Glutamate synthases (GOGAT) were analyzed to identify the functional binding domains of the substrate (glutamine) and cofactors (FMN, NAD(P)H, FAD, [3Fe-4S] and [4Fe-4S] clusters and ferredoxin) on this enzyme. The published amino acid sequences of six different NAD(P)Hdependent GOGATs (NAD(P)H-GOGAT) and ten different ferredoxin-dependent GOGATs (Fd-GOGAT) were used for this analysis. The amin...

Journal: :Biochemical Society transactions 2007
N G Karagüler R B Sessions B Binay E B Ordu A R Clarke

Enzymes have become important tools in several industries due to their ability to produce chirally pure and complex molecules with interesting biological properties. The NAD(+)-dependent LDH (lactate dehydrogenase) [bsLDH [Geobacillus stearothermophilus (formerly Bacillus stearothermophilus) LDH] from G. stearothermophilus and the NAD(+)-dependent FDH (formate dehydrogenase) [cmFDH (Candida met...

Journal: :Applied and environmental microbiology 1998
C Kujo T Ohshima

NAD-dependent glutamate dehydrogenase (L-glutamate:NAD oxidoreductase, deaminating; EC 1.4.1.2) was purified to homogeneity from a crude extract of the continental hyperthermophilic archaeon Pyrobaculum islandicum by two successive Red Sepharose CL-4B affinity chromatographies. The enzyme is the most thermostable NAD-dependent dehydrogenase found to date; the activity was not lost after incubat...

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