نتایج جستجو برای: na k
تعداد نتایج: 617134 فیلتر نتایج به سال:
This study was conducted to evaluate the effect of salinity on concentration of potassium (K), sodium (Na) and chloride (Cl) ions, in sweet lime budded on five citrus rootstocks, including Bakraii (mandarin x sweet lime), Volcameriana, Sour orange, Sweet lime and Mexican lime in a glasshouse, using a randomized completely design with factorial arrangement and four replications. Rootstocks had...
This study was conducted to evaluate the effect of salinity on concentration of potassium (K), sodium (Na) and chloride (Cl) ions, in sweet lime budded on five citrus rootstocks, including Bakraii (mandarin x sweet lime), Volcameriana, Sour orange, Sweet lime and Mexican lime in a glasshouse, using a randomized completely design with factorial arrangement and four replications. Rootstocks had...
In order to evaluate the effect of seed priming on salinity modification of safflower (cv. Goldasht), a hydroponic experiment was conducted at Research Greenhouse of Vali-e-Asr University of Rafsanjan, Iran, in 2009. Priming treatments at 4 levels (no priming as control, and priming with distilled water, NaCl and Ca(NO3)2 for 24 hours) and salinity treatments at 4 levels (0, 8, 16 and 24...
Mutations in the RS1 gene cause X-linked juvenile retinoschisis (XLRS), a hereditary retinal dystrophy. We recently showed that retinoschisin, the protein encoded by RS1, regulates ERK signaling and apoptosis in retinal cells. In this study, we explored an influence of retinoschisin on the functionality of the Na/K-ATPase, its interaction partner at retinal plasma membranes. We show that retino...
The effects of [Na(+)] or [K(+)] on Na, K-ATPase activity of FW-acclimated and SW-acclimated tilapia, puffer and milkfish were examined in gill homogenates. [Na(+)] or [K(+)] stimulated Na, K-ATPase hydrolyzing ATP in all experimental groups. ATP hydrolysis stimulated by [Na(+)] or [K(+)] followed Michaelian-Menten kinetics. Km values for [K(+)] (i.e., Km(K)), were lower in SW- than FW-acclimat...
This study was designed to identify and characterize Na+-activated K+ current (I(K(Na))) in guinea pig gastric myocytes under whole-cell patch clamp. After whole-cell configuration was established under 110 mM intracellular Na+ concentration ([Na+]i) at holding potential of -60 mV, a large inward current was produced by external 60 mM K+([K+]degrees). This inward current was not affected by rem...
Changes in Na(+)/K(+)-ATPase activity and abundance associated with environmental salinity were investigated in the gills and rectal gland of the Atlantic stingray Dasyatis sabina. Using a ouabain-specific ATPase assay and western blotting, we found that stingrays from fresh water had the highest activity and highest relative abundance of Na(+)/K(+)-ATPase in the gills. Using immunohistochemist...
Decades ago, it was proposed that Na transport in cardiac myocytes is modulated by large changes in cytoplasmic Na concentration within restricted subsarcolemmal spaces. Here, we probe this hypothesis for Na/K pumps by generating constitutive transsarcolemmal Na flux with the Na channel opener veratridine in whole-cell patch-clamp recordings. Using 25 mM Na in the patch pipette, pump currents d...
Ouabain-sensitive uncoupled Na+ efflux has been studied in human, pig, and rat red cells and in vesicles containing reconstituted kidney Na+/K+ pumps obtained from these same species. The red cells from the different species gave qualitatively similar results; the uncoupled Na+ efflux was 15-30% of the Na+/K+ exchange rate, and this flux was inhibited at 5 mM extracellular Na+ (Na+o). At higher...
Red blood cells exposed to ouabain are capable of net Na outflux against an electrochemical gradient; the net outflux is inhibited by the diuretic, furosemide. In ouabain-treated cells, both the unidirectional Na outflux and the unidirectional Na influx are inhibited by furosemide. Furosemide also inhibits the ouabain-sensitive Na-Na exchange accomplished by the Na-K pump in K-free solutions. F...
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