The synthesis of heterologous proteins in Lactococcus lactis is strongly influenced by the promoter selected for the expression. The nisin A promoter is commonly used for induced expression of proteins in L. lactis, whereas few constitutive promoters (P45 and the weaker P32) have been used for protein expression studies. In this study, eight different putative strong constitutive promoters were...

Computing the topology of an algebraic plane curve C means to compute a combinatorial graph that is isotopic to C and thus represents its topology in R2. We prove that, for a polynomial of degree n with coefficients bounded by 2ρ , the topology of the induced curve can be computed with Õ(n8(n+ρ2)) bit operations deterministically, and with Õ(n8ρ2) bit operations with a randomized algorithm in e...

Here, we use a time change of the processes N7(·) and N5(·) to construct service completions with cross-selling candidates that are followed by actual cross-selling. These two processes are used if the threshold is non-negative. In this case, a service completion is followed by a cross-selling offer whenever the queue is equal to 0 or when it is greater than 0 but smaller than the threshold. We...

This work treats degenerate four-wave mixing ~DFWM! in noncentrosymmetric materials, taking into full account the fact that the DFWM signal arises from third-order nonlinear optical effects as well as from two distinct combinations of second-order effects: second-harmonic generation plus difference frequency generation and optical rectification plus Pockels effect. Because of these second order...

We obtained monoclonal antibodies against N(1),N(12)-diacetylspermine (DiAcSpm) and N(1),N(8)-diacetylspermidine (DiAcSpd), and developed two systems of competitive ELISA that utilize the antibodies and a common enzyme-labeled antigen to measure these di-acetylpolyamines. Cross-reactions with N(1)-acetylspermidine in the assay of DiAcSpm and with N8-acetylspermidine in the assay of DiAcSpd were...

The Quadratic Assignment Problem (QAP) is the problem of making exclusive assignments of n indivisible entities to n other indivisible entities in such a way that a total quadratic interaction cost is minimized. The problem can be interpreted from a wide variety of perspectives. The perspective adopted in this paper is that of the generic facilities location/layout context, as in the seminal wo...

Alert correlation is a very useful mechanism to reduce the high volume of reported alerts and to detect complex and coordinated attacks. Existing approaches either require a large amount of expert knowledge or use simple similarity measures that prevent detecting complex attacks. They also suffer from high computational issues due, for instance, to a high number of possible scenarios. In this p...

In this paper, density functional theory (DFT) study was applied to the absorption of BMSF-BENZ ((4-bromo-7-methoxy-1-(2-methoxyethyl)-5-{[3 (methylsulfonyl)phenyl]methyl)}-2-[4-(propane-2)) yl)phenyl]-1H-1,3-benzothiazole) used for treatment Osteoporosis, on surfaces two types nanocages Zn12O12 and Al12P12. The results showed that interaction with highly exothermic, indicating high chemical ad...

An increasing number of new subtypes avian influenza viruses (AIVs) are reported to be infecting humans, including H3N2, H5N1, H7N9, H10N8, and the recently emerged H3N8 virus in China 2022. However, genetic biological properties currently prevalent AIVs not yet fully understood. This study reports isolation a novel triple reassortment (GD-H3N8) from chicken flocks Guangdong province, China, Th...

Saeki, Tomoaki, Jian-Bing Shen, and Achilles J. Pappano. Carbachol promotes Na1 entry and augments Na/Ca exchange current in guinea pig ventricular myocytes. Am. J. Physiol. 273 (Heart Circ. Physiol. 42): H1984–H1993, 1997.— The effect of carbachol (CCh) on the Na/Ca exchange current (INa/Ca) was studied in voltage-clamped ventricular myocytes isolated from guinea pig hearts and superfused with...