objective: crispr/cas9 technology provides a powerful tool for targeted modification of genomes. in this system, a donor dna harboring two flanking homology arms is mostly used for targeted insertion of long exogenous dna. here, we introduced an alternative design for the donor dna by incorporation of a single short homology arm into a circular plasmid. materials and methods: in this experiment...

purpose: to evaluate exposure rate following wrapping hydroxyapatite implant in mersilene mesh and donor sclera. methods: in a prospective comparative interventional case-series 60 consecutive patients underwent primary hydroxyapatite orbital implant wrapped in mersilene mesh or donor sclera. results: thirty patients (21 male and 10 female) underwent implantation with mersilene mesh and 30 pati...

background some grafts harvested from the groin area do not become hyperpigmented and in an individual with multiple pieces of grafts, the hyperpigmentation of the pieces may vary. this study evaluates the orientation of the inset of groin grafts according to their donor sites (superior-inferior) and its role in graft hyperpigmentation. methods patients with hand burn contracture or syndactyly ...

The optical properties of two Re(CO)3(bpy)Cl complexes in which the bpy is substituted with donor (triphenylamine, TPA, ReTPA2) as well both (TPA) and acceptor (benzothiadiazole, BTD, ReTPA-BTD) groups are presented. For ReTPA2 absorption spectra show intense intraligand charge-transfer (ILCT) bands at 460 nm small solvatochromic behavior; for ReTPA-BTD ILCT transitions weaker. These assigned T...