Plant virus-based nanoparticles (VNPs) offer a bioinspired approach to the delivery of drugs and imaging agents. The chemical addressability, biocompatibility, scalable manufacturability VNPs make them promising alternative synthetic platforms. However, VNPs, just like other proteinaceous or (NPs), are readily recognized cleared by immune system mechanisms such as opsonization phagocytosis. Shi...

PEGylated and surface-tethered proteins are used in a variety of biotechnological applications, but traditional methods offer little control over the placement functionalization sites on protein. Fortunately, recent experimental functionalize protein at any location amino acid sequence, so question becomes one selecting site that will result best function. This work shows how molecular simulati...

BSA and lysozyme have been transformed into macroinitiators for living radical polymerisation and used to produce well-defined bioconjugates which can be fluorescently labelled providing a versatile strategy for the preparation of bioconjugates which is complementary to traditional PEGylation.

A strained aza-dibenzocyclooctyne was prepared via a high-yielding synthetic route. Copper-free, strain-promoted click reaction with azides showed excellent kinetics, and a functionalised aza-cyclooctyne was applied in fast and efficient PEGylation of enzymes.

An efficient and bench-stable reagent was synthesized for direct and covalent introduction of tetrazines onto target protein or virus surfaces, which can be further modified based on tetrazine-ene ligation to achieve fluorescence labelling or PEGylation under mild conditions.

introduction: it is well known that glycoconjugates on the cell surface of embryonic cells as well as extracellular matrix (ecm) are involved in many developmental phenomena including cell differentiation and maturation. these molecular events have not received proper attention during pituitary cell differentiation. the purpose of this study was to investigate glycoconjugate distribution on cel...

Prophylaxis with 2-4 times weekly dosing of factor (F)VIII or FIX is established as an efficacious and safe treatment in haemophilia. Although prophylaxis is not readily available for the inhibitor patient, recent studies have demonstrated a reduction in bleeding episodes in inhibitor patients treated with daily infusions of FVIIa. In order to develop a treatment option comparable to prophylaxi...

Interaction with platelets has been recognized as a concern in the development of liposomeencapsulated hemoglobin as an oxygen carrier. This reaction causes a significant drop in circulating platelets in a rapid and transient fashion. We studied the effect of lipid bilayer charge on the magnitude of drop in circulating platelets. We also investigated the effect of post-inserted PEG-lipid on thi...

One disadvantage of vesicular stomatitis virus G (VSV-G) pseudotyped lentivirus vectors for clinical application is inactivation of the vector by human serum complement. To prevent this, monomethoxypoly(ethylene) glycol was conjugated to a VSV-G-human immunodeficiency virus vector expressing Escherichia coli beta-galactosidase. The modification did not affect transduction efficiency in vitro an...

AIMS To develop and determine the safety of gold nanorods, whose aspect ratios can be tuned to obtain plasmon peaks between 650 and 850 nm, as contrast enhancing agents for diagnostic and therapeutic applications. MATERIALS & METHODS In this study we compared the blood clearance and tissue distribution of cetyl trimethyl ammonium bromide (CTAB)-capped and polyethylene glycol (PEG)-coated gold...