Abstract In this study, liquid smoke production waste gas (LSPW) was used as a substitute for carbon monoxide (CO) and filter in handling frozen tuna steaks. Tuna steaks were packed plastic bags, then inside the bag, steak sprayed with CO LSPW, stored at 4°C two days. After that, each package vacuumed -25°C four weeks. Observation of quality deterioration started from beginning storage until fo...

Recently great advances have been made in the preparation of pure myoglobin by the introduction of ion exchange cellulose column chromatography (1, 2). Myoglobin, however, shows a strong tendency to be oxidized to metmyoglobin during isolation procedures. For this reason all myoglobin preparations reported earlier were obtained in the form of metmyoglobin. Therefore, oxymyoglobin has usually be...

Objective: The objective of this study was to determine the changes in myoglobin denaturation through cooking three different muscles medium rare, medium, or well-done degrees doneness.

Since the first description by Theorell (11) of a process of isolating and purifying myoglobin from horse heart by crystallization, several workers have succeeded in preparing myoglobin by Theorell’s procedure. Roche and Vieil (8) and Rossi and Aragona (9) have described methods less arduous than Theorell’s. Their methods, however, sacrifice yield to gain simplicity. We have developed a modific...

Myoglobin is a globular protein involved in oxygen storage and transport. No consensus yet exists on the atomic level mechanism by which oxygen and other small nonpolar ligands move between the myoglobin's buried heme, which is the ligand binding site, and surrounding solvent. This study uses room temperature molecular dynamics simulations to provide a complete atomic level picture of ligand mi...

Myoglobin levels were assayed in each urine specimen voided during 12 hours before and 48 hours after routine cardiac catheterisation in 146 patients using an indirect haemagglutination method detecting concentrations in excess of 0.015 mg/ml. Myoglobinuria was found in only one patient before but in 39 patients after cardiac catheterisation (27%), either in the first (34 patients) or the secon...

Iron chelators such as desferrioxamine have been shown to ameliorate oxidative damage in vivo. The mechanism of this therapeutic action under non-iron-overload conditions is, however, complex, as desferrioxamine has properties that can impact on oxidative damage independent of its capacity to act as an iron chelator. Desferrioxamine can act as a reducing agent to remove cytotoxic ferryl myoglob...

A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) using two monoclonal antibodies directed against a synthetic peptide with an amino-acid sequence related to the C-terminus of bovine myoglobin and the whole molecule of sodium dodecyl sulphate (SDS)-denatured bovine myoglobin was adapted for detecting bovine myoglobin in contaminated feeds. The ELISA employed bovine meat ext...

A quantitative and dynamic analysis of skeletal muscle structure and function can guide training protocols and optimize interventions for rehabilitation and disease. While technologies exist to measure body composition, techniques are still needed for quantitative, long-term functional imaging of muscle at the bedside. We evaluate whether diffuse optical spectroscopic imaging (DOSI) can be used...

Myoglobin is an intracardiac protein that is released into the blood after myocardial injury and is then cleared rapidly by the kidneys. This study was undertaken to determine whether successful reperfusion of damaged myocardium could be assessed by examination of blood myoglobin concentration-time patterns. After release of a 2 hr occlusion of the mid left anterior descending coronary artery i...