Polypeptide Ags present in the culture filtrate of Mycobacterium tuberculosis were purified and evaluated for their ability to stimulate PBMC from purified protein derivative (PPD)-positive healthy donors. One such Ag, which elicited strong proliferation and IFN-gamma production, was further characterized. The N-terminal amino acid sequence of this polypeptide was determined and used to design ...

A new mycobacteriophage-based technique (PhageTek MB) was compared with standard culture and staining techniques for diagnosis of pulmonary tuberculosis. A total of 2,048 respiratory specimens from 1,466 patients collected from February 2000 to March 2001 were studied by both (i) conventional methods (direct microscopic examination [auramine-rhodamine fluorochrome], and culture in BacT/ALERT 3D...

1 van Embden JDA, Cave MD, Crawford JT, et al. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standard methodology. J Clin Microbiol 1993;31:406–9. 2 Ruddy MC, Davies AP, Yates MD, et al. Outbreak of isoniazid resistant Mycobacterium tuberculosis in north London. Thorax 2004;59:279–85. 3 Drobniewski FA, Watterson SA, Wilson SM, et al. A clinical...

1 van Embden JDA, Cave MD, Crawford JT, et al. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standard methodology. J Clin Microbiol 1993;31:406–9. 2 Ruddy MC, Davies AP, Yates MD, et al. Outbreak of isoniazid resistant Mycobacterium tuberculosis in north London. Thorax 2004;59:279–85. 3 Drobniewski FA, Watterson SA, Wilson SM, et al. A clinical...

Tuberculosis (TB) is one of the most important AIDS associated infectious diseases worldwide. It is a leading cause of illness and death among people with HIV/AIDS in resource-poor areas of the world. The annual incidence of TB among indigenous Iranians stands at 14 cases per 100,000 inhabitants. This study aimed to identify Mycobacterium infection among Iranian HIV positive patients. Two sputu...

Tuberculosis in seals is caused by Mycobacterium pinnipedii, a member of the Mycobacterium tuberculosis complex. In this study, we evaluated the extent of genetic variability among Mycobacterium bovis and M. pinnipedii by microarray-based comparative genomics. We identified two deletions that are exclusive to M. pinnipedii: PiD1 that removes the orthologues of the M. tuberculosis genes Rv3530c ...

Pulmonary tuberculosis is rampant in some countries. The disease an outcome of infection by Mycobacterium and more common immunocompromised individuals. Furthermore, mycetoma or a fungal ball can develop cavitary lesions tuberculosis. present case rare presentation pulmonary with concomitant Aspergillus diabetic Indian male. A clinical examination strong laboratory radiological workup helped es...

INTRODUCTION Bacterial infections may appear as sequelae of remote tuberculous infections, especially thoracic infections. The simultaneous appearance of tuberculosis and bacterial infection is not common, and, to our knowledge, the association of infection by Streptococcus anginosus and Mycobacterium tuberculosis has not been reported previously in the literature. CASE PRESENTATION We report...