نتایج جستجو برای: multiplex rt
تعداد نتایج: 80305 فیلتر نتایج به سال:
Untreated groundwater is responsible for about half of the waterborne disease outbreaks in the United States. Human enteric viruses are thought to be leading etiological agents of many of these outbreaks, but there is relatively little information on the types and levels of viruses found in groundwater. To address this problem, monthly samples from 29 groundwater sites were analyzed for 1 year ...
Avian bornaviruses were first described in 2008 as the causative agents of proventricular dilatation disease (PDD) parrots and their relatives (Psittaciformes). To date, 15 genetically highly diverse avian covering at least five viral species have been discovered different bird orders. Currently, primary diagnostic tool is detection RNA by conventional or real-time RT-PCR (rRT-PCR). One drawbac...
The paper describes a developed comprehensive system for molecular-genetic identification of Yersinia pestis strains according to their appurtenance certain subspecies, biovars, phylo-geographic populations, using realtime PCR (RT-PCR), allele-specific RT-PCR, and multiplex with hybridization fluorescent registration results on solid substrate. Application this makes it possible establish the Y...
Accurate and rapid diagnosis of Pandemic Influenza A/H1N1 2009 virus (H1N1 2009) infection is important for the prevention and control of influenza epidemics and the timely initiation of antiviral treatment. This study was conducted to evaluate the performance of several diagnostic tools for the detection of H1N1 2009. Flocked nasopharyngeal swabs were collected from 254 outpatients of suspecte...
Gene expression measurements detailing mRNA quantities are widely employed in molecular biology and are increasingly important in diagnostic fields. Reverse transcription (RT), necessary for generating complementary DNA, can be both inefficient and imprecise, but remains a quintessential RNA analysis tool using qPCR. This study developed a Transcriptomic Calibration Material and assessed the RT...
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